Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line

CHEN Hongyan; et al

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Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line

更新时间：2024-01-17

- Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line
- Journal of Chongqing Medical University Issue 3, Pages: 369-372(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学基础医学院
  3. 重庆医科大学附属儿童医院耳鼻喉科
  4. 汕头大学医学院,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.65
- Published：2006，
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[1]陈鸿雁,王驰,舒艳,陈燕,吕彩凤,余晓燕,沈娜,张潜英,叶玲,蒋纪恺.抗肿瘤药物与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响研究[J].重庆医科大学学报,2006(03):369-372.

CHEN Hongyan, et al. Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line. [J]. Journal of Chongqing Medical University (3):369-372(2006)
[1]陈鸿雁,王驰,舒艳,陈燕,吕彩凤,余晓燕,沈娜,张潜英,叶玲,蒋纪恺.抗肿瘤药物与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响研究[J].重庆医科大学学报,2006(03):369-372. DOI：

CHEN Hongyan, et al. Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line. [J]. Journal of Chongqing Medical University (3):369-372(2006) DOI：

摘要

目的:探讨6种常用抗肿瘤药物长春新碱、阿霉素、平阳霉素、顺铂、5-Fu、环磷酰胺与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响。方法:以喉癌KBV200细胞株为研究对象

通过流式细胞术检测6种抗肿瘤药物作用KBV200细胞的P-糖蛋白表达以及联合维拉帕米对P-糖蛋白表达的影响。结果:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用

使KBV200细胞的P-糖蛋白表达下降80.17%、89.69%、19.19%及53.48%。其中维拉帕米与长春新碱、阿霉素作用较强。结论:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用后

P-糖蛋白的表达水平明显下降

对KBV200细胞耐药性的逆转有一定作用。

Abstract

Objective: To observe the effect of verapamil and 6 chemotherapeutic agents vincristine

adriamycin

bleomycin

cyclophosphamide

5-Fu

cisplatin on the P-glucoprotein(P-gp) of the KBV200 cell line.Methods: Flow cytometer(FCM) assay was used to investigate the expression of P-gp on the KBV200 cell line in the different conditions.Results: Verapamil act with vincristine

adriamycin

pingyangmycin and cyclophosphamide separately decreased expression of P-gp on KBV200 cell line by 80.17%

89.69%

19.19% and 53.48% Among all the combinations

the synergistic effects of varapamil act with vincristine and adriamycine show a better effect.Conclusion: Verapamil act with vincristine

adriamycin

pingyangmycin and cyclophosphamide separately can decrease expression of P-gp on KBV200 cell line significantly

and can reverse the drug resistance of KBV200 cell line.

关键词

KBV200细胞株多药耐药耐药逆转P糖蛋白维拉帕米

Keywords

KBV200 multidrug resistance cell linemultidrug resistanceReversion of multidrug resistanceP-gpVerapamil

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