The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro

WANG Guanghui; et al

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The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro

更新时间：2024-01-17

- The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro
- Journal of Chongqing Medical University Issue 6, Pages: 851-853(2006)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学基础医学院病理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2006，
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[1]王光辉,王顺和,杨慧敏,李苏,李苒,谭晓姝.大鼠NSCs向多巴胺能神经元分化研究[J].重庆医科大学学报,2006(06):851-853+915.

WANG Guanghui, et al. The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro. [J]. Journal of Chongqing Medical University (6):851-853(2006)
[1]王光辉,王顺和,杨慧敏,李苏,李苒,谭晓姝.大鼠NSCs向多巴胺能神经元分化研究[J].重庆医科大学学报,2006(06):851-853+915. DOI：

WANG Guanghui, et al. The effects of IL-1 beta on the differentiation of rats NSCs into dopaminergic neurons in vitro. [J]. Journal of Chongqing Medical University (6):851-853(2006) DOI：

摘要

目的:探讨IL-1β对神经干细胞（NSCs）分化为多巴胺能神经元作用。方法:体外培养和鉴定中脑来源NSCs

用免疫学方法检测分化细胞酪氨酸羟化酶（TH）表达。结果:血清组、IL-1β10pg/ml组、IL-1β120pg/ml组、IL-1β150pg/ml组、IL-1β200pg/ml组诱导7d后TH细胞分别平均为0.45%、0.6%、7.2%、12.5%、8.75%。结果显示IL-1β诱导NSCs明显提高TH细胞表达率

与血清组比较有显著性差异（P<0.05）

在IL-1β150pg/ml剂量范围内TH细胞表达率与剂量呈正相关。结论:IL-1β有诱导NSC向多巴胺能神经元分化的显著作用。

Abstract

Objective:To explore the effects of IL-1 beta on the differentiation of NSCs into dopaminergic neurons.Methods: To isolate and culture mesencephalic neural stem cells in vitro.we used immunocytochemistry to detect TH-positive neurons induced by 10%FBS and interleukin-1 bate for 7days respectively.Results: The positive ratio of TH expression in induced cells by 10%FBS

IL-1β 10pg/ml

IL-1β 120pg/ml

IL-1β 150pg/ml

IL-1β 200pg/ml were 0.45%

0.6%

7.2%

12.5%and 8.75% respectively.The positive ratios of TH expression in induced cells by IL-1β 150pg/ml(12.5%) were much higher than that by 10%FBS(control 0.45%)(P<0.05)

The positive ratio of TH expression in induced cells was relate to the dosage of IL-1β.Conclusion:The induction of IL-1 beta on the differentiation of NSCs into dopaminergic neurons in vitro is significant.

关键词

神经干细胞分化白细胞介素-1β多巴胺能神经元

Keywords

Neural stem cells(NSCs)differentiationIL-1 betaDopaminergic neurons

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Department of Neurology,the Children’s Hospital,Chongqing Medical University

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Department of Child Health Care, College of Pediatrics, Chongqing Medical University

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