Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG

WANG Hong; et al

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Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG

更新时间：2024-01-17

- Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG
- Journal of Chongqing Medical University Issue 3, Pages: 299-301(2006)
- 作者机构：
  
  1. 重庆医科大学传染病寄生虫病研究所
  2. 重庆医科大学传染病寄生虫病研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2006，
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[1]王鸿,李文桂.多房棘球绦虫Em14-3-3抗原编码基因在BCG中的表达[J].重庆医科大学学报,2006(03):299-301.

WANG Hong, et al. Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG. [J]. Journal of Chongqing Medical University (3):299-301(2006)
[1]王鸿,李文桂.多房棘球绦虫Em14-3-3抗原编码基因在BCG中的表达[J].重庆医科大学学报,2006(03):299-301. DOI：

WANG Hong, et al. Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG. [J]. Journal of Chongqing Medical University (3):299-301(2006) DOI：

摘要

目的:研究多房棘球绦虫Em14-3-3抗原编码基因在BCG中的表达。方法:将重组质粒pBCG-Em14-3-3用电穿孔法导入BCG构建rBCG

将含有重组子的细菌培养至对数生长期

在收菌前3天每天45℃热诱导30min

然后对表达产物作SDS-PAGE及免疫印迹分析。结果:多房棘球绦虫pBCG-Em14-3-3在BCG中成功表达了Em14-3-3重组蛋白

在相对分子量为27KDa处可见明显的表达蛋白带

其表达量占菌体总蛋白量的11%。结论:多房棘球绦虫pBCG-Em14-3-3能在BCG中高效表达

且能与鼠血清抗体发生特异结合

提示rBCG-Em14-3-3疫苗表达的Em14-3-3重组蛋白具有特异的抗原性。

Abstract

Objective: To study the expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in Bacillus Calmette-Guerin(BCG).Methods: The recombinant plasmid pBCG-Em14-3-3 was introduced into BCG by electroporation and the bacteria with pBCG-Em14-3-3 were cultured to logarithm growth period

then induced daily by heating at 45℃ for 30min in the last three days.The expression products were analyzed by SDS-PAGE and Western blot.Results: The Em14-3-3 recombinant protein was successfully expressed by pBCG-Em14-3-3 in BCG as an obvious band of 27kDa by SDS-PAGE

which accounted for 11% of total bacterial protein in BCG.Conclusion: The pBCG-Em14-3-3 could be expressed efficiently in BCG and be combined with specific antibody in mice sera

which suggested that the Em14-3-3 recombinant protein expressed by rBCG-Em14-3-3 vaccine was of specific antigenicity.

关键词

多房棘球绦虫重组Em14-3-3蛋白BCG基因表达

Keywords

Echinococcus multilocularisRecombinant Em14-3-3 protein.BCGgene expression

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