Establishment of two-dimensional polyacrylamide gel electrophoresis for serum proteomics

MU Zhaode; et al

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Establishment of two-dimensional polyacrylamide gel electrophoresis for serum proteomics

更新时间：2024-01-17

- Establishment of two-dimensional polyacrylamide gel electrophoresis for serum proteomics
- Journal of Chongqing Medical University Issue 6, Pages: 821-823(2006)
- 作者机构：
  
  1. 重庆医科大学药学院药物分析教研室
  2. 重庆医科大学生物化学与分子生物学教研室
  3. 重庆医科大学医学检验系,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.1
- Published：2006，
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[1]母昭德,彭咏波,易发平,邱宗荫.血清蛋白质组双向凝胶电泳技术的建立[J].重庆医科大学学报,2006(06):821-823+840.

MU Zhaode, et al. Establishment of two-dimensional polyacrylamide gel electrophoresis for serum proteomics. [J]. Journal of Chongqing Medical University (6):821-823(2006)
[1]母昭德,彭咏波,易发平,邱宗荫.血清蛋白质组双向凝胶电泳技术的建立[J].重庆医科大学学报,2006(06):821-823+840. DOI：

MU Zhaode, et al. Establishment of two-dimensional polyacrylamide gel electrophoresis for serum proteomics. [J]. Journal of Chongqing Medical University (6):821-823(2006) DOI：

摘要

目的:建立血清蛋白质组双向凝胶电泳（2-DE）技术。方法:对血清蛋白质2-DE的各种条件进行调整、优化。结果:建立了稳定的2-DE技术。结论:已建立的血清蛋白质2-DE技术

将为进一步开展疾病的血清蛋白质组学研究奠定基础。

Abstract

Objective: To establish a two-dimensional polyacrylamide gel electrophoresis(2-DE) technique for serum proteomics.Methods: Various conditions of serum proteomic 2-DE were adjusted and optimized.Results: A steady 2-DE technique was established.Conclusions: Our established 2-DE technique of serum proteins can be effectively applied in serum proteomics of diseases.

关键词

血清蛋白质组学蛋白质组双向凝胶电泳

Keywords

Serum proteomicsProteomeTwo-dimensional polyacrylamide gel electrophoresis(2-DE)

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