Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells

您当前的位置：

首页 >

文章列表页 >

Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells

更新时间：2024-01-17

|

- Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells
- Journal of Chongqing Medical University Issue 3, Pages: 307-310(2006)
- 作者机构：
  
  1. 第三军医大学预防医学系复合伤研究所
  2. 第三军医大学第三附属医院神内科
  3. 第三军医大学第一附属医院急诊科
  4. 第三军医大学预防医学系复合伤研究所,重庆,400038
  5. ,重庆,400042
- 作者简介：
- 基金信息：
- DOI：
  CLC： R641
- Published：2006，
扫描看全文
[1]江德鹏,向静,文亮,粟永萍,屈纪富,冉新泽.白细胞蛋白酶抑制因子对真皮间充质干细胞增殖的影响[J].重庆医科大学学报,2006(03):307-310+329.

JIANG Depeng, et al. Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells. [J]. Journal of Chongqing Medical University (3):307-310(2006)
[1]江德鹏,向静,文亮,粟永萍,屈纪富,冉新泽.白细胞蛋白酶抑制因子对真皮间充质干细胞增殖的影响[J].重庆医科大学学报,2006(03):307-310+329. DOI：

JIANG Depeng, et al. Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells. [J]. Journal of Chongqing Medical University (3):307-310(2006) DOI：

摘要

目的:研究白细胞蛋白酶抑制因子（SLPI）在真皮间充质干细胞（dMSCs）中的表达以及促dMSCs增殖的作用。方法:采用前期建立的技术分离、纯化并扩增真皮多能干细胞

在大鼠背部置入聚乙烯酒精海绵收集伤后1天伤口液

用Tripure细胞裂解液提取伤口液刺激前后dMSCs和创伤前后大鼠皮肤组织中总RNA。通过RT-PCR检测伤口液刺激前后dMSCs和创伤前后大鼠皮肤组织中SLPImRNA的表达。用M-PER抽提细胞蛋白

了解伤口液刺激前后dMSCs中SLPI蛋白表达的变化。无血清IMDM培养液培养细胞48h

加入不同浓度的SLPI因子刺激dMSCs

通过[H3]Thymidine掺入实验研究SLPI对真皮间充干细胞的增殖作用。结果:分离的大鼠dMSCs呈梭形

在体外显示多向分化潜能。RT-PCR和Westernblot提示SLPI在dMSCs中表达

伤口液刺激后表达增强。通过[H3]Thymidine掺入实验发现加入SLPI因子后

细胞增殖明显增强。结论:SLPI在dMSCs中表达

能够促进dMSCs的增殖

通过高表达SLPI可能是dMSCs参与创伤修复的重要途径之一。

Abstract

Objective:To investigate the expression of SLPI in dMSCs and the effect of SLPI on growth regulaton of dMSCs.Method :Isolated stem cells from the rat skin tissue.Two pieces of sterile polyvinyl alcohol sponges were inserted subcutaneously in the dorsum of the rat and collected the wound fluid 1day after injury.Isolated RNA of two groups:dMSCs before and after the stimulation by wound fluid;skin tissue before and after trauma.SLPI was detected to know how they changed in dMSCs and in the skin before and after the injure by using RT-PCR and Western blot method.[H3] Thymidine incorporation was used to evaluate the mitogenic influence of SLPI.Results:Most of the neonatal dMSCs were spindle-shaped cells and exhibited multilineage potential in vitro.It was shown in the result of RT-PCR and western blot that SLPI expressed in dMSCs and increased after the cells being stimulated by wound fluid.A concentration-dependent proliferation promotion were demonstrated in dMSCs incubated for 24h in the prsense of SLPI.Conclusions:SLPI can be detected in dMSCs and support the growth of dMSCs in serum-free medium in vitro.In view of the multiple functions of SLPI

we supposed that it acted as an important role when dMSCs participated in the reparing of cutaneous injury.

关键词

白细胞蛋白酶抑制因子干细胞增殖

Keywords

Secretory leukocyte protease inhibitorStem cellsproliferation

references

0

Views

56

下载量

0

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Proteasome activator subunit 1/2 promotes the proliferation and invasion of endometrial carcinoma cells by inhibiting the expression of cyclin-dependent kinase 15

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Research progress of molecular mechanism of palmitic acid-induced pancreatic β-cell dysfunction

Association of NUSAP1 with tumor cell proliferation and immune cell infiltration in esophageal carcinoma: database based research

Related Author

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Zhang Hailin

Li Cong

Xiao Zhenghua

Liao Juan

Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Obstetrics and Gynecology,Yongchuan Hospital Affiliated to Chongqing Medical University

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University

Central Laboratory of Yongchuan Hospital Affiliated to Chongqing Medical University

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

⁰