MicroRNA depresse the expression of MAD2 gene in trophoblastic cells

SHI Qiong; et al

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MicroRNA depresse the expression of MAD2 gene in trophoblastic cells

更新时间：2024-01-17

- MicroRNA depresse the expression of MAD2 gene in trophoblastic cells
- Journal of Chongqing Medical University Issue 2, Pages: 138-141(2007)
- 作者机构：
  
  1. 重庆医科大学基础医学院放射医学教研室
  2. 重庆医科大学临床检验诊断学实验室
  3. 重庆医科大学临床检验诊断学实验室重庆医科大学临床检验诊断学实验室
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R346
- Published：2007，
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[1]施琼,翁亚光,徐远久,蔡燕,蒋洪彦.微小RNA抑制胚胎细胞MAD2基因的表达[J].重庆医科大学学报,2007(02):138-141.

SHI Qiong, et al. MicroRNA depresse the expression of MAD2 gene in trophoblastic cells. [J]. Journal of Chongqing Medical University (2):138-141(2007)
[1]施琼,翁亚光,徐远久,蔡燕,蒋洪彦.微小RNA抑制胚胎细胞MAD2基因的表达[J].重庆医科大学学报,2007(02):138-141. DOI：

SHI Qiong, et al. MicroRNA depresse the expression of MAD2 gene in trophoblastic cells. [J]. Journal of Chongqing Medical University (2):138-141(2007) DOI：

摘要

目的:探讨微小RNA（microRNA）抑制胚胎细胞MAD2基因表达的机理。方法:用定量real-time PCR比较microRNA转染前后的胚胎细胞内源性基因MAD2 mRNA表达水平

同时用western blot比较转染前后Mad2蛋白的表达水平。结果:对照组的MAD2基因mRNA拷贝数/GAPAH mRNA拷贝数为0.1780±0.0688

而转染microRNA重组质粒后

实验组的mRNA比值分别为0.1778±0.0689和0.1778±0.0670

经统计分析

两实验组细胞的内源性MAD2基因mRNA水平与对照组无显著性差异;而其中一实验组的蛋白水平在转染后有明显降低。结论:microRNA主要在翻译水平调节哺乳动物细胞内基因的表达

为研究基因表达的调控机制奠定了良好的基础。

Abstract

Objective:To discuss the mechanism of down-regulated expression of MAD2 gene in trophoblastic cells by microRNA.Methods:Endogenous MAD2 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid;and the Mad2 protein level was compared by western blot.Results:Ratio of MAD2 mRNA/GAPDH mRNA of control group was 0.1780±0.0688

and after transfected with microRNA recombinant plasmid was 0.1778±0.0689 and 0.1778±0.0670respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level in one of the experimental groups was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can down-regulate gene expression in mammalian cells at translation level

it is a good foundation to study on the control of gene expression.

关键词

微小RNAMAD2基因定量RT-PCR

Keywords

microRNAMAD2 geneQuantitative real-time PCR

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徐远久

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Related Institution

重庆医科大学临床检验诊断学实验室基础医学院核医学教研室

Laboratory of Clinical Diagnostics,Chongqing Medical University

感染性疾病分子生物学教育部重点实验室

Clinical Medical Research Center，the Second Clinical Medical College of Jinan UniversityShenzhen People’s Hospital

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