Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As<sub>2</sub>O<sub>3</sub>） in human renal cell carcinoma 786-0 cells

WANG Delin; et al

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Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As₂O₃） in human renal cell carcinoma 786-0 cells

更新时间：2024-01-17

- Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As₂O₃） in human renal cell carcinoma 786-0 cells
- Journal of Chongqing Medical University Issue 2, Pages: 134-137(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学基础医学院病理教研室
  3. 重庆医科大学附属第一医院泌尿外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2007，
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[1]王德林,米粲,陈在贤,吴小候,苟欣.As_2O_3抑制人肾癌786-0细胞血管生成及浸润转移机制的初步研究[J].重庆医科大学学报,2007(02):134-137.

WANG Delin, et al. Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As₂O₃） in human renal cell carcinoma 786-0 cells. [J]. Journal of Chongqing Medical University (2):134-137(2007)
[1]王德林,米粲,陈在贤,吴小候,苟欣.As_2O_3抑制人肾癌786-0细胞血管生成及浸润转移机制的初步研究[J].重庆医科大学学报,2007(02):134-137. DOI：

WANG Delin, et al. Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As₂O₃） in human renal cell carcinoma 786-0 cells. [J]. Journal of Chongqing Medical University (2):134-137(2007) DOI：

摘要

目的:探讨三氧化二砷（As2O3）抑制人肾癌细胞系786-0细胞血管生成及浸润转移的机制。方法:采用免疫细胞化学方法检测As2O3处理前后786-0细胞VEGF、nm23、MMP-9、TIMP-1蛋白表达的改变;RT-PCR分析TGF-β1、TβR-I和TβR-IImRNA表达的改变。结果:2μmol/L As2O3处理肾癌786-0细胞72h后可显著抑制VEGF、MMP-9的蛋白表达（P<0.01）

而促进786-0细胞中TIMP-1和nm23蛋白的表达（P<0.05）。786-0细胞中缺乏TβR-I mRNA

As2O3处理后

786-0细胞中TGF-β1和TβR-II mRNA表达无改变（P>0.05）。结论:As2O3通过抑制VEGF和MMP-9的蛋白表达

增加TIMP-1和nm-23蛋白的表达

抑制786-0细胞的血管生成和转移。

Abstract

Objective:To explore the mechanisms of angiogenesis

invasion and metastasis inhibited by arsenic trioxide(As2O3)in human renal cell carcinoma 786-0 cells.Methods:The changes of VEGF

nm23

MMP-9

and TIMP-1 protein expression were studied by immunocytochemistry

and TGF-β1

TβR-I and TβR-II mRNA were analysed by reverse transcription polymerase chain reaction in 786-0 cells treated with As2O3.Results:2μmol/L As2O3 effectively inhibited the protein expression of VEGF and MMP-9 in 786-0 cells(P<0.05)

but increased nm23 and TIMP-1 protein expression(P<0.05).Absence of expression of TβR-ImRNA was found in 786-0 cells

and no change of mRNA expression of TGF-β1 and TβR-II was detected in 786-0 cells treated with As2O3 for 72h.Conclusions As2O3 might inhibit the angiogenesis

invasion and metastasis of 786-0 cells by down-regulating the expression of VEGF and MMP-9 protein and up-regulating nm23 and TIMP-1 protein.

关键词

As2O3肾癌786-0细胞血管生成转移

Keywords

Arsenic trioxiderenal cell carcinoma786-0 cellsangiogenesismetastasis

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Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As2O3） in human renal cell carcinoma 786-0 cells

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摘要

Abstract

关键词

Keywords

references

Mechanisms of angiogenesis,invasion and metastasis inhibited by arsenic trioxide（As₂O₃） in human renal cell carcinoma 786-0 cells