Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat

ZHOU Chenglin; et al

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Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat

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- Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat
- Journal of Chongqing Medical University Issue 6, Pages: 794-797(2006)
- 作者机构：
  
  1. 重庆医科大学药学院
  2. 重庆医科大学检验系
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q51-3
- Published：2006，
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[1]周成林,王继生,李惠芝,邱宗荫,宋敏.大鼠脑组织蛋白质双向电泳技术的建立[J].重庆医科大学学报,2006(06):794-797.

ZHOU Chenglin, et al. Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat. [J]. Journal of Chongqing Medical University (6):794-797(2006)
[1]周成林,王继生,李惠芝,邱宗荫,宋敏.大鼠脑组织蛋白质双向电泳技术的建立[J].重庆医科大学学报,2006(06):794-797. DOI：

ZHOU Chenglin, et al. Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat. [J]. Journal of Chongqing Medical University (6):794-797(2006) DOI：

摘要

目的:建立大鼠脑组织蛋白质双向电泳技术。方法:利用不同体积的裂解液提取大鼠脑组织中的蛋白质

并通过不同的蛋白质上样量（1mg

2mg

3mg）

进行双向电泳

考马斯亮蓝染色

图谱分析。结果:在等电点3～10

分子量6.5～200ku范围内分离得到蛋白质斑点为

1mg蛋白质上样量为546个蛋白质斑点

2m g为780个

3m g为805个斑点。2mg蛋白质上样量的双向电泳图谱更清晰

分离更好。结论:成功建立了大鼠脑组织蛋白质的双向电泳技术。

Abstract

Objective:To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods:Lysis buffer of different volume was taken to extract brain tissue proteins of rat

and different protein quantities(1mg

2mg

3mg) were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein

and patterns were analyzed.Results:With a molecular mass between 6.5~200ku and isoelectric points(pI) from 3~10

1mg proteins obtained 546 protein spots

2mg 780

and 3mg 805.Pattern of 2mg protein was the best.Conclusion:A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.

关键词

蛋白质组学双向电泳脑组织裂解液

Keywords

proteomicsTwo-dimensional electrophoresisBrain tissueLysis buffer

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重庆医科大学药学院

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