Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia

CHEN Lifen; et al

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Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia

更新时间：2024-01-17

- Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia
- Journal of Chongqing Medical University Issue 2, Pages: 153-155(2006)
- 作者机构：
  
  1. 重庆医科大学附属第二医院神经内科
  2. 重庆医科大学基础医学院病理生理教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R743.3
- Published：2006，
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[1]陈莉芬,胡长林,汤为学.阿魏酸钠对培养大鼠大脑微血管内皮细胞缺氧损伤的保护作用[J].重庆医科大学学报,2006(02):153-155.

CHEN Lifen, et al. Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia. [J]. Journal of Chongqing Medical University (2):153-155(2006)
[1]陈莉芬,胡长林,汤为学.阿魏酸钠对培养大鼠大脑微血管内皮细胞缺氧损伤的保护作用[J].重庆医科大学学报,2006(02):153-155. DOI：

CHEN Lifen, et al. Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia. [J]. Journal of Chongqing Medical University (2):153-155(2006) DOI：

摘要

目的:研究缺氧对脑微血管内皮细胞（brain microvascular endothelial cells

BMEC）的损伤及阿魏酸钠（Sodium feru-late）对它的保护作用。方法:取体外培养的大鼠脑皮质微血管内皮细胞

置于95%N2和5%CO2的缺氧盒中12h造成内皮细胞缺氧。用透射电镜观察BMEC的超微结构变化。用内皮细胞线粒体活性和内皮细胞死亡率来评价内皮细胞活力

并用乳酸脱氢酶（LDH）漏出量作为评价损伤的指标。结果:体外培养大鼠脑皮质BMEC缺氧12h后

细胞超微结构损伤

细胞存活率及线粒体活性明显降低

LDH释放量显著增加。阿魏酸钠可显著对抗缺氧对BMEC的损伤。结论:阿魏酸钠对大鼠脑皮质BMEC缺氧损伤具有保护作用。

Abstract

Objective: To study the protective effect of Sodium ferulate on rat brain microvascular endothelial cells(BMEC) injured by hypoxia.Methods: BMEC from Wistar rat cerebral cortex was cultured and placed in an anoxia chamber with 95%N2 and 5% CO2 for 12 hours.BMEC activity or injury was assayed by observing cell ultramicrostructures and measuring viability by methyl thiazolyl tetrazolium(MTT) assay

trypan blue staining(TBST) and the lactate dehydrogenase(LDH)

a marker of cell death.Results: The BMEC activity and viability was decreased after hypoxia as assessed by MTT

TBST and LDH

The ultrastructure of BMEC was damaged after hypoxia.SF could enhance BMEC activity and viability and ameliorate hypoxia injury.Conclusions: Sodium ferulate exerts protective action on BMEC injured by hypoxia.

关键词

脑微血管内皮细胞缺氧阿魏酸钠

Keywords

Brain microvascular endothelial cellshypoxiaSodium ferulate

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