Ex vivo expansion of human cord blood CD<sub>34</sub><sup>+</sup> cells with hematopoietic growth factors

WANG Jianwei; et al

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Ex vivo expansion of human cord blood CD₃₄⁺ cells with hematopoietic growth factors

更新时间：2024-01-17

- Ex vivo expansion of human cord blood CD₃₄⁺ cells with hematopoietic growth factors
- Journal of Chongqing Medical University Issue 2, Pages: 148-152(2006)
- 作者机构：
  
  1. 重庆医科大学组织学与胚胎学教研室
  2. 重庆医科大学组织学与胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2006，
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[1]王建伟,王亚平,王莎莉,姜蓉.脐血CD_(34)~+细胞的体外扩增研究[J].重庆医科大学学报,2006(02):148-152.

WANG Jianwei, et al. Ex vivo expansion of human cord blood CD₃₄⁺ cells with hematopoietic growth factors. [J]. Journal of Chongqing Medical University (2):148-152(2006)
[1]王建伟,王亚平,王莎莉,姜蓉.脐血CD_(34)~+细胞的体外扩增研究[J].重庆医科大学学报,2006(02):148-152. DOI：

WANG Jianwei, et al. Ex vivo expansion of human cord blood CD₃₄⁺ cells with hematopoietic growth factors. [J]. Journal of Chongqing Medical University (2):148-152(2006) DOI：

摘要

目的:探讨造血生长因子不同组合方式对脐血CD34+造血干/祖细胞（HSC/HPC）的扩增作用。方法:应用StemsepTM阴性分选系统分离纯化CD34+细胞

在体外液体培养体系中经不同组合细胞因子进行扩增1￣3w。结果:FL+Tpo+SCF+IL-3+GM-CSF+G-CSF+Epo组合的扩增效果最佳

可分别扩增细胞总数、造血祖细胞集落总数（CFCs）、CD34+细胞达1627.57±52.93、56.78±8.13、41.09±4.11倍。FL和Tpo具有明显的协同扩增早期祖细胞的作用

CFCs和CD34+细胞在1￣2w维持在较高水平

以后逐渐衰退。结论:合理的细胞因子组合在大量扩增造血细胞的同时也有助于早期造血祖细胞的自我更新

把握适宜的扩增时机

将有助于扩增细胞在质和量上满足移植和基因治疗等的要求。

Abstract

Objective: To investigate the role of recombinant human hematopoietic growth factors(HGFs) in expansion of purified CD34+ cells derived from human cord blood.Methods: A sterilized separation system for purification of CD34+ hematopoietic stem / progentior cells(HSC/HPC) was established by means of StemsepTM according to the strategy of negative selection on immunomagnetic separation.CD34+ cells were expanded with various combination of cytokines in a 21-day liquid culture systems.Results: A combination of seven HGFs including FL

Tpo

SCF

IL-3

GM-CSF

G-CSF and Epo was identified as the most potent combination of those tested

and it increased total nucleated cell by 1627.57±52.93 fold

total progenitor cells(CFCs) by 56.78±8.13 fold and CD34+ cell by 41.09±4.11 fold

respectively.Among the HGFs examined

FL and Tpo were the most potent ones for expanding cord blood CD34+ cells.Maximal expansions of CFCs and CD34+ cells were observed during the first two weeks of culture.Conclusion: Under conditions of appropriate HGFs combination and expansion duration

it is possible to expand cord blood CD34+ cells ex vivo for transplantation and therapeutic gene transfer.

关键词

脐血造血干/祖细胞造血生长因子扩增

Keywords

Human cord bloodCD34+ cellsHematopoietic growth factorExpansion

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重庆医科大学基础医学研究所

Department of Histology and Embryology,College of Basic Medicine,Chongqing Medical University

重庆医科大学基础医学院组胚教研室

Department of Histology and Embryology,College of Basic Medicine,Chongqing Medical University

⁰

Ex vivo expansion of human cord blood CD34+ cells with hematopoietic growth factors

DOI：

摘要

Abstract

关键词

Keywords

references

Ex vivo expansion of human cord blood CD₃₄⁺ cells with hematopoietic growth factors