Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus

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Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus

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- Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus
- Journal of Chongqing Medical University Issue 1, Pages: 1-3(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院传染病寄生虫病研究所
  2. 重庆医科大学附属第一医院传染病寄生虫病研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2007，
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[1]李文桂,朱佑明,王鸿.细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后IgG及其亚类和IgE的动态观察[J].重庆医科大学学报,2007(01):1-3+26.

LI Wengui, et al. Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus. [J]. Journal of Chongqing Medical University (1):1-3(2007)
[1]李文桂,朱佑明,王鸿.细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后IgG及其亚类和IgE的动态观察[J].重庆医科大学学报,2007(01):1-3+26. DOI：

LI Wengui, et al. Dynamic observation on IgG,its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus. [J]. Journal of Chongqing Medical University (1):1-3(2007) DOI：

摘要

目的:动态观察细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后IgG及其亚类和IgE的变化。方法:将疫苗分别采用鼻腔内接种和口服灌胃免疫BALB/C鼠

在免疫后0、2、4、6、8、10、12、14、16和18周各剖杀4只小鼠

眼球取血

常规ELISA测定血清中IgG及其亚类和IgE水平

同时设有PBS对照。结果:鼻腔内接种组的血清IgG、IgG2a和IgG2b水平均在免疫后2￣18周升高

分别在免疫后10、4和6周达最高水平;血清IgG1、IgG3和IgE水平均在免疫后2￣18周降低

分别在免疫后8、12和10￣12周达最低水平。口服免疫组的血清IgG、IgG2a和IgG2b水平均在免疫后2￣18周增加

分别在免疫后14、8和8周达峰值;血清IgG1、IgG3和IgE水平均在免疫后2￣18周下降

分别在免疫后8、6和16￣18周达最低水平。结论:细粒棘球绦虫重组BCG-Eg95疫苗在免疫早期即可诱导小鼠产生一个TH1型保护性免疫反应。

Abstract

Objective:To dynamically observe changes of IgG

its subclass and IgE in sera of mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).Methods:BALB/C mice were intranasally or orally vaccinated and killed on 0、2、4、6、8、10、12、14、16 and 18w of immunization

with 4 in each killing.Sera were gathered from the eyeball to measure IgG

its subclass and IgE by routine ELISA

with PBS as control.Results:In the intranasal group

levels of IgG、IgG2a and IgG2b increased obviously on 2~18w

and reached the highest level on 10、4 and 6w respectively;while levels of IgG1、IgG3 and IgE decreased remarkably on 2~18w

and came to the lowest level on 8、12 and 10~12w respectively.In the oral group

levels of IgG、IgG2a and IgG2b rose remakably on 2~18w

and came to the peak value on 14、8 and 8w respectively;while levels of IgG1、IgG3 and IgE decreased on 2~18w

and reached the lowest level on 8、6 and 16~18w respectively.Conclusion:TH1 response could be induced in mice immunized by recombinant BCG-Eg95 vaccine of Echinococcus granulosus in early immunization.

关键词

细粒棘球绦虫重组BCG-Eg95疫苗IgG及其亚类IgE

Keywords

Echinococcus granulosusRecombinant BCG-Eg95 vaccineIgG and its subclassIgE

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