Construction of mouse peritonitis models of Enterococcus

WU Lixian; et al

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Construction of mouse peritonitis models of Enterococcus

更新时间：2024-01-17

- Construction of mouse peritonitis models of Enterococcus
- Journal of Chongqing Medical University Issue 1, Pages: 4-7(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院感染科
  2. 重庆医科大学附属第一医院感染科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R-332
- Published：2007，
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[1]吴利先,黄文祥,孙小平.肠球菌小鼠腹膜炎模型的建立[J].重庆医科大学学报,2007(01):4-7.

WU Lixian, et al. Construction of mouse peritonitis models of Enterococcus. [J]. Journal of Chongqing Medical University (1):4-7(2007)
[1]吴利先,黄文祥,孙小平.肠球菌小鼠腹膜炎模型的建立[J].重庆医科大学学报,2007(01):4-7. DOI：

WU Lixian, et al. Construction of mouse peritonitis models of Enterococcus. [J]. Journal of Chongqing Medical University (1):4-7(2007) DOI：

摘要

目的:建立肠球菌毒力相关性小鼠腹膜炎模型

用于肠球菌类毒力因子的研究。方法:用粪肠球菌标准毒株OG1RF和粪肠球菌sprE基因缺陷突变株来建立小鼠腹膜炎模型

通过腹膜炎指标的检测来判断毒力下降情况。结果:突变株在小鼠腹膜炎模型中的半数致死量（LD50）提高了7倍

存活率也明显高于标准株。6h和12h外周血白细胞变化低于标准株

6h腹水TNF-α水平和活化TNF-α低于标准株组。结论:肠球菌毒力相关性小鼠腹膜炎模型建立成功

可用于肠球菌其它可能毒力因子的研究。

Abstract

Objective:To construct mouse peritonitis models of Enterococcus for the study of the putative virulence factor of Enterococcus.Methods:We used the standard strain OG1RF and sprE gene deficient mutant of Enterococcus faecalis to construct mouse peritonitis models.And the index of peritonitis was detected to measure the virulence decrease of sprE mutant. Results:It was showed that 50%lethal dose(LD50)of sprE mutant was 7 times than that of standard strain OG1RF

and its survival rate was also prominently that of OG1RF in the mouse peritonitis model;while the change of blood leukocytes of sprE mutant group was lower than that of standard stain at 6 hour and 12 hour;So were the TNF-α level in peritoneal fluid and active TNF-α in peritonitis at 6 hour.Conclusion:The peritonitis models of Enterococcus was constructed successfully

which would be used to study other putative virulence factor of Enterococcus face-ali.

关键词

肠球菌腹膜炎模型毒力

Keywords

EnterococcusMouse peritonitis modelsVirulence

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Department of Infectious Diseases,the First Affiliated Hospital,Chongqing Medical University

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