Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure

KE Dan; et al

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Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure

更新时间：2024-01-17

- Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure
- Journal of Chongqing Medical University Issue 6, Pages: 615-618(2007)
- 作者机构：
  
  1. 重庆医科大学生物医学工程系/医学超声工程研究所
  2. 重庆市第一人民医院皮肤科
  3. 重庆医科大学生物医学工程系/医学超声工程研究所,重庆,400016
  4. ,重庆,400011
- 作者简介：
- 基金信息：
- DOI：
  CLC： R454
- Published：2007，
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[1]柯丹,李发琪,刁庆春,朱小娟,刘春梅,龚晓波.低强度脉冲超声波促进皮肤成纤维细胞增殖的实验研究[J].重庆医科大学学报,2007(06):615-618.

KE Dan, et al. Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure. [J]. Journal of Chongqing Medical University (6):615-618(2007)
[1]柯丹,李发琪,刁庆春,朱小娟,刘春梅,龚晓波.低强度脉冲超声波促进皮肤成纤维细胞增殖的实验研究[J].重庆医科大学学报,2007(06):615-618. DOI：

KE Dan, et al. Proliferation of cultured skin fibroblast after low intensity pulsed ultrasound exposure. [J]. Journal of Chongqing Medical University (6):615-618(2007) DOI：

摘要

目的:研究不同辐照参数（频率、声强、辐照时间）低强度脉冲超声波（LIPUS）对成纤维细胞增殖的影响。方法:采用MTT法测定频率1MHz、1.2MHz、1.7MHz

每一频率下四种声强的LIPUS

同一声强分别辐照5min、10min、15min、20min和30min体外培养的人真皮成纤维细胞24h后的生长增殖情况

筛选出促进成纤维细胞生长的最适宜频率、声强和辐照时间。结果:①在MTT法测定细胞活性中三种频率（1MHz、1.2MHz、1.7MHz）

每一频率下四种声强的LIPUS辐照成纤维细胞5min

24h后吸光光度值均增加

辐照成纤维细胞10min

24h后吸光光度值增加最高

较对照组都有显著性差异（P<0.01）。超过一定辐照时间后

吸光光度值下降

且随着辐照时间的延长

吸光光度值下降显著。②频率1MHz、声强0.1W/cm2、辐照时间10min下的细胞相对成活率最高

与对照组比较差异有统计学意义（P<0.01）。结论:不同频率、不同声强的LIPUS辐照一定时间均能促进人真皮成纤维细胞增殖

超过一定辐照时间则会抑制细胞生长。而频率1MHz、声强0.1W/cm2、辐照时间10min为促进成纤维细胞增殖较为适宜的辐照参数。

Abstract

Objective:To observe the effects of different low intensity pulsed ultrasound(LIPUS) on proliferation of fibroblast and to screen out the optimum parameters of LIPUS.Methods:LIPUS of different frequency(1?1.2?1.7MHz)and different intensity was used to stimulate the cultured human fibroblast cells for 5 min

10 min

20 min and 30min

respectively

and examination of cell proliferation by MTT after 24h.Results:①Optical absorption value of cell activity in MTT assay increased significantly in the cell culture with LIPUS of different frequency(1?1.2?1.7MHz) and four intensity of each frequency treatment for 5

10 minutes

as compared with the control group(P<0.01).With a peak detected in the cell culture with LPUS treatment for 10 minutes.But optical absorption value decreased with longer treat time

optical absorption value decreased significantly in human skin fibroblasts with LIPUS stimulation for more than 30 minutes(P<0.01)

as compared with the control group.②The peak relative survival rate detected in the cell culture with LIPUS of 1MHz

0.1W/cm2 treatment for 10 minutes.Conclusion:Stimulation with LIPUS could induce cell proliferation at a shorter time and could inhibit cell growth in vitro at a longer time.1MHz

0.1W/cm2 treatment for 10 minutes is the better parameter for promote cell proliferation.

关键词

低强度脉冲超声波成纤维细胞增殖

Keywords

Low intensity pulsed ultrasoundfibroblastproliferation

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