Effects of GDNF gene-activated matrix on neuron protection after sciatic nerve injury in rats

LIANG Anlin; et al

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Effects of GDNF gene-activated matrix on neuron protection after sciatic nerve injury in rats

更新时间：2024-01-17

- Effects of GDNF gene-activated matrix on neuron protection after sciatic nerve injury in rats
- Journal of Chongqing Medical University Issue 9, Pages: 930-934(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院骨科
  2. 重庆医科大学附属第一医院骨科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.3
- Published：2007，
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[1]梁安霖,蒋电明,安洪.GDNF基因活化的细胞外基质对大鼠坐骨神经损伤后神经元的保护作用[J].重庆医科大学学报,2007(09):930-934.

LIANG Anlin, et al. Effects of GDNF gene-activated matrix on neuron protection after sciatic nerve injury in rats. [J]. Journal of Chongqing Medical University (9):930-934(2007)
[1]梁安霖,蒋电明,安洪.GDNF基因活化的细胞外基质对大鼠坐骨神经损伤后神经元的保护作用[J].重庆医科大学学报,2007(09):930-934. DOI：

LIANG Anlin, et al. Effects of GDNF gene-activated matrix on neuron protection after sciatic nerve injury in rats. [J]. Journal of Chongqing Medical University (9):930-934(2007) DOI：

摘要

目的:探讨GDNF基因活化的细胞外基质对神经元的保护作用。方法:通过化学萃取获得大鼠坐骨神经细胞外基质

复合上编码GDNF的质粒DNA

构建成基因活化的细胞外基质支架。60只成年Wistar大鼠按随机数字法分为3组:A组（n=20）GDNF基因活化的细胞外基质桥接组

B组（n=20）ECM桥接组

C组（n=20）自体神经桥接组

术后3天、1周、4周、12周时用RT-PCR、激光共聚焦显微镜等形态学方法来评价神经元保护作用。结果:GDNF基因活化的细胞外基质能作为局部的基因缓释系统来释放GDNF

在脊髓中高效表达12周以上。GDNF mRNA的表达和Nissl染色阳性细胞的数量在脊髓中明显增加

而iNOS表达明显下降。神经元存活率明显优于对照组（78.04%±3.50%vs 56.09%±1.89%）。结论:GDNF基因活化的细胞外基质可以促进GDNF mRNA在脊髓中表达

增加前角运动神经元的存活率

其机制可能与iNOS受抑制有关。

Abstract

Objective:To evaluate the effects of glial cell line-derived neurotrophic factor(GDNF) gene-activated matrix(GAM) in protection of neurons after sciatic nerve injury.Methods:The peripheral nerve extracellular matrix was harvested by chemical extraction.GDNF GAM was comprised of the extracellular matrix and plasmids encoding GDNF.Sixty adult Wistar rats were randomly divided into three groups:group A(GDNF GAM conduits

n=20)

group B(ECM conduits

n=20)

group C(autografts

n=20).Lumbar enlargement was processed for Nissl staining

immunohistochemistry and RT-PCR for GDNF or iNOS and fluorescence observation.Fluorescence observation was used to confirm the expressing of GDNF in the spinal cord.Motoneurons(MN) of lesioned(L) and normal(N) sides were counted

and MN survival ratio(MSR=L/N) was calculated.Results:GDNF DNA can be retained in GAM

promoting transgene expressing in axotomized neurons in spinal cord for 12 weeks.In GAM group

the expression of GDNF mRNA was maintained for 12 weeks while the number of iNOS positive cells was decreased in comparison with ECM group.GAM grafts obviously increased the survival rate of motor neurons than that of ECM grafts(78.04%±3.50% vs 56.09%±1.89%) at 12 weeks postaxotomy.Conclusion:These results suggest that(1) GDNF GAM increases the GDNF mRNA and the amount of Nissl positive cells in the lumber enlargement;(2) GDNF GAM protects MN through a mechanism in relation to iNOS.

关键词

胶质细胞源性神经营养因子细胞外基质坐骨神经损伤修复大鼠神经元

Keywords

Glial cell line-derived neurotrophic factorExtracellular matrixSciatic nerve injury and repairRaneuron

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