Study of biological properties of bone marrow stromal cells in vitro in wistar rats

YANG Qin; et al

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Study of biological properties of bone marrow stromal cells in vitro in wistar rats

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- Study of biological properties of bone marrow stromal cells in vitro in wistar rats
- Journal of Chongqing Medical University Issue 9, Pages: 926-929(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院神经内科
  2. 重庆医科大学附属第一医院影像科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2007，
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[1]杨琴,曾志磊,谢鹏,杨军,吕发金.体外培养大鼠骨髓基质细胞生物学特性研究[J].重庆医科大学学报,2007(09):926-929+934.

YANG Qin, et al. Study of biological properties of bone marrow stromal cells in vitro in wistar rats. [J]. Journal of Chongqing Medical University (9):926-929(2007)
[1]杨琴,曾志磊,谢鹏,杨军,吕发金.体外培养大鼠骨髓基质细胞生物学特性研究[J].重庆医科大学学报,2007(09):926-929+934. DOI：

YANG Qin, et al. Study of biological properties of bone marrow stromal cells in vitro in wistar rats. [J]. Journal of Chongqing Medical University (9):926-929(2007) DOI：

摘要

目的:观察体外培养大鼠骨髓基质细胞生物学特性

为诱导骨髓基质细胞分化形成神经元及分化机制和移植治疗脑缺血大鼠的研究奠定基础。方法:取4周龄Wistar大鼠

全骨髓法体外培养骨髓基质细胞

利用倒置相差显微镜观察原代及不同传代细胞生长形态特点

并应用间接免疫荧光法鉴定原代及不同传代细胞表型。结果:培养的骨髓基质细胞第3代

细胞增殖能力已趋于稳定

骨髓基质细胞表面标志CD54、CD44阳性的细胞已占到95.18%、94.36%。造血干细胞表面标志CD14阳性的细胞已从原代的23.19%降低到2.37%。结论:传3代的细胞已适合于细胞诱导分化

可作为理想细胞移植治疗的细胞来源。

Abstract

Objective:To observe the biological properties of bone marrow stromal cells(MSCs) in vitro in Wistar rats for inducing MSCs to differentiate into neurons and transplantating MSCs to treat cerebral ischemic stroke.Methods:The Wistar rats for 4 weeks old were sacrified

the bone marrow separated and the MSCs cultured

and the biological properties of the cells observed by phasecontrast microscope.Cell surface markers were evaluated by indirect immunofluorescence cycochemistry staining in primary and different generation cells.Results:95.18% and 94.36% of the third generation cells showed positivity to CD54 and CD44(MSCs surface marker).The percentage of hematopoietic lineage marker CD14 decreased obviously from 23.19% in primal culture of the cells to 2.37% in the third generation.Conclusion:The third generation cells can be used as the seed-cells for study of cells induce and differentiation and the resoure of cells transplantation.

关键词

Wistar大鼠骨髓基质细胞细胞培养

Keywords

Wistar ratsBone marrow stromal cellscell culture

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