Objective:To establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tissure. Methods:Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. Results:Under the culture condition in vitro

newborn rat neurons showed similar properties to their counterparts in vivo and typical nerver fiber net was formed. Conclusion:This technique for the primary culture of cortical neurons is an ideal good technique for the general labs.