The primary study about the relationship of FBN2 gene and lung cancer cell lines

CHEN Hong

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The primary study about the relationship of FBN2 gene and lung cancer cell lines

更新时间：2024-01-17

- The primary study about the relationship of FBN2 gene and lung cancer cell lines
- Journal of Chongqing Medical University Issue 11, Pages: 1175-1177(2007)
- 作者机构：
  
  重庆医科大学附属第一医院呼吸内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2007，
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[1]陈虹.FBN2基因与肺癌细胞关系的初步探讨[J].重庆医科大学学报,2007(11):1175-1177+1195.

CHEN Hong. The primary study about the relationship of FBN2 gene and lung cancer cell lines. [J]. Journal of Chongqing Medical University (11):1175-1177(2007)
[1]陈虹.FBN2基因与肺癌细胞关系的初步探讨[J].重庆医科大学学报,2007(11):1175-1177+1195. DOI：

CHEN Hong. The primary study about the relationship of FBN2 gene and lung cancer cell lines. [J]. Journal of Chongqing Medical University (11):1175-1177(2007) DOI：

摘要

目的:探讨FBN2基因在肺癌细胞中的表达及其受甲基化的调控。方法:培养肺癌细胞株HCC366、H1299、H2195和人正常支气管上皮细胞（NHBEC）

并用5-Aza-cdR干预。用逆转录方法观察FBN2基因的表达

并用MSP检测是否存在FBN2的异常甲基化状态。结果:①FBN2基因在HCC366、H1299不表达

但在NHBEC、H2195中表达。②经过5-Aza-cdR处理后

FBN2基因在HCC366、H1299可以重新表达。③HCC366、H1299细胞存在FBN2基因的异常甲基化表现现象。结论:FBN2基因在HCC366、H1299细胞中不表达

但5-Aza-cdR能使其重新表达。FBN2基因沉默可能是受启动子异常甲基化调控的影响。

Abstract

Objective:To study the gene expression and aberrant methylation of FBN2 on lung cancer cell lines.Methods:Three lung cancer cell lines HCC366

H1299 and H2195 were cultured.mRNA was analysed by RT-PCR and methylation status was also investigated by methylation specific PCR.The loss of FBN2 expression cell lines were treated with the demethylating agent

5-aza-2’-deoxycytid-ine(5-aza-cdR).Results:The expression of FBN2 was detected in NHBEC and H2195

whereas it was not detected in HCC366 and H1299 which showed aberrant methylation of FBN2.FBN2 expression was restored after treatment with 5-aza-cdR.Conclusion:Methylation and silencing of FBN2 in tumor cells may play an important role in carcinogenesis of lung cancer.

关键词

FBN2肺癌细胞甲基化5-Aza-cdR

Keywords

FBN2Lung cancer cell linesmethylation5-Aza-cdR

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Related Institution

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Department of Cardiology,Laboratory of Children’s Cardiovascular Disease,Ministry of Education Key Laboratory of Child Development and Disorders,the Children’s Hospital of Chongqing Medical University

Department of Cardiothoracic Surgery, the First Affiliated Hospital, Chongqing Medical University

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