Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro

WANG Gaiqin; et al

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Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro

更新时间：2024-01-17

- Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro
- Journal of Chongqing Medical University Issue 11, Pages: 1178-1181(2007)
- 作者机构：
  
  1. 重庆医科大学基础医学院组胚教研室
  2. 重庆医科大学基础医学院组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2007，
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[1]王改琴,杜小丽,吴宏.细胞密度和血清对小鼠骨髓基质细胞体外生长的影响[J].重庆医科大学学报,2007(11):1178-1181+1222.

WANG Gaiqin, et al. Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro. [J]. Journal of Chongqing Medical University (11):1178-1181(2007)
[1]王改琴,杜小丽,吴宏.细胞密度和血清对小鼠骨髓基质细胞体外生长的影响[J].重庆医科大学学报,2007(11):1178-1181+1222. DOI：

WANG Gaiqin, et al. Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro. [J]. Journal of Chongqing Medical University (11):1178-1181(2007) DOI：

摘要

目的:研究不同细胞接种密度、血清浓度及种类对小鼠骨髓基质细胞体外生长的影响。方法:设置不同细胞密度、不同种类血清及血清量的培养体系

观察细胞贴壁情况

细胞80%贴壁时间

用台盼蓝排斥试验检测活细胞率

MTT法检测细胞增殖情况

HE染色光镜下观察细胞形态。结果:原代全血培养的小鼠骨髓基质细胞适宜接种密度为1～4×106/ml;细胞最适生长的血清浓度为10%～20%;与胎牛血清相比马血清对小鼠骨髓基质细胞的促增殖作用更好（P<0.05）;不同种类血清培养的小鼠骨髓基质细胞类型有一定差异（P<0.05）。结论:不同细胞接种密度及血清浓度和血清种类对小鼠骨髓基质细胞体外生长有一定影响。

Abstract

Objective:To study the effect of different cell culture densities and sera on growth of mice bone marrow stromal cells in vitro.Methods:Bone marrow stromal cells were cultured in DMEM/F12 containing different cell densities and sera.The rate of adhered cells

cell viability by trypan blue test

proliferation of bone marrow stromal cells by MTT and cell morphological structure with light microscope were observed.Results:The cultured cells proliferated well in DMEM/F12 containing 10%～20% sera and the densities of 1～4×106/ml.It prolifrated better in horse serum than fetal bovine serum(P<0.05).Its types were also different in newbone calf serum

fetal bovine serums and horse serum(P<0.05).Conclusion:Different cell densities and sera has influence on the growth of mice bone marrow stromal cells.

关键词

小鼠骨髓基质细胞生长体外培养

Keywords

Bone marrow stromal cellsgrowthIn vitro culture

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