Effect of different pneumoperitoneums on the growth of human cervical carcinoma cells in vitro

GAN Xiaoqin; et al

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Effect of different pneumoperitoneums on the growth of human cervical carcinoma cells in vitro

更新时间：2024-01-17

- Effect of different pneumoperitoneums on the growth of human cervical carcinoma cells in vitro
- Journal of Chongqing Medical University Issue 3, Pages: 261-263(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院妇产科
  2. 重庆医科大学附属第一医院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2007，
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[1]干晓琴,姚珍薇,王华斌.腹腔镜不同气腹介质对人宫颈癌细胞株CaSki体外生长影响的研究[J].重庆医科大学学报,2007(03):261-263+321.

GAN Xiaoqin, et al. Effect of different pneumoperitoneums on the growth of human cervical carcinoma cells in vitro. [J]. Journal of Chongqing Medical University (3):261-263(2007)
[1]干晓琴,姚珍薇,王华斌.腹腔镜不同气腹介质对人宫颈癌细胞株CaSki体外生长影响的研究[J].重庆医科大学学报,2007(03):261-263+321. DOI：

GAN Xiaoqin, et al. Effect of different pneumoperitoneums on the growth of human cervical carcinoma cells in vitro. [J]. Journal of Chongqing Medical University (3):261-263(2007) DOI：

摘要

目的:探讨腹腔镜不同气腹介质对人宫颈癌细胞株CaSki体外生长的影响。方法:体外培养CaSki细胞

模拟腹腔镜建立CO2气腹和N2气腹处理细胞

另设未处理组为对照。采用MTT法、流式细胞术及电镜比较各组CaSki细胞的生长情况。结果:处理后48h起CO2促进CaSki细胞生长

N2组在处理后48￣72h短暂抑制细胞增殖;CO2组G2-M期细胞明显增加;CO2组细胞见明显核分裂像

N2组可见线粒体肿胀

核周间隙扩大。结论:CO2气腹促进CaSki细胞生长

而N2气腹则起一定的抑制作用。

Abstract

Objective:To evaluate the effects of different pneumoperitoneums used in laparoscopy on the growth of human cervical carcinoma cells in vitro.Methods:Human cervical carcinoma cell line(CaSki)were cultured in vitro and then exposed to pneumoperitoneums of pure carbon dioxide or pure nitrogen gas with the untreated group as control. The cell growth was determined by MTT assay

flow cytometry and electron microscopy. Results:Carbon dioxide stimulated the cell growth from 48h to 120h after treatment. For nitrogen gas group

there was a transient descent of cell proliferation from 48h to 72h after treatment. The tumor cells of carbon dioxide group in G2-M phases significantly increased. Active karyokinesis was observed in carbon dioxide group while slight mitochondrion swelling was observed in nitrogen gas group with the nucleus space expanded. Conclusions:Carbon dioxide pneumoperitoneum could increase the growth of human cervical carcinoma cells in vitro whereas nitrogen gas pneumoperitoneum had inhibiting effect on the cell growth.

关键词

腹腔镜二氧化碳N2气腹宫颈肿瘤体外生长

Keywords

laparoscopyCarbon dioxideNitrogen gaspneumoperitoneumCervical neoplasmCell growth in vitro

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