Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat

HAO Jie; et al

您当前的位置：

首页 >

文章列表页 >

Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat

更新时间：2024-01-17

- Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat
- Journal of Chongqing Medical University Issue 3, Pages: 236-238(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院科研处
  2. 重庆医科大学公共卫生学院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2007，
扫描看全文
[1]郝杰,王应雄,何俊琳,刘学庆,刘孝云,陈雪梅,黎刚.人参皂甙Rg1对镉诱导的大鼠睾丸损伤的保护作用[J].重庆医科大学学报,2007(03):236-238.

HAO Jie, et al. Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat. [J]. Journal of Chongqing Medical University (3):236-238(2007)
[1]郝杰,王应雄,何俊琳,刘学庆,刘孝云,陈雪梅,黎刚.人参皂甙Rg1对镉诱导的大鼠睾丸损伤的保护作用[J].重庆医科大学学报,2007(03):236-238. DOI：

HAO Jie, et al. Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat. [J]. Journal of Chongqing Medical University (3):236-238(2007) DOI：

摘要

目的:探讨人参皂甙Rg1对镉诱导的大鼠睾丸损伤的保护作用及作用机制。方法:30只雄性wistar大鼠随机分为对照组、氯化镉组和Rg1+氯化镉组。氯化镉组大鼠按0.5mg（/kg·d）氯化镉腹腔染毒

Rg1+氯化镉组大鼠在0.5mg（/kg·d）氯化镉腹腔染毒2h前进行按100mg（/kg·d）Rg1灌胃处理

连续14天后将动物处死

分离附睾

测定精子数量和质量。分离睾丸

测定金属硫蛋白（MT）含量

TUNEL法检测睾丸切片凋亡情况。结果:氯化镉引起附睾中精子数量减少

质量降低

金属硫蛋白表达增加

睾丸中精细胞凋亡指数增加

而Rg1可以改善附睾中精子质量和数量

减少精细胞凋亡指数

并进一步上调金属硫蛋白表达。结论:人参皂甙Rg1对镉诱导的大鼠睾丸损伤具有保护作用

机制可能与上调金属硫蛋白表达有关。

Abstract

Objective:To investigate the protective effect and the possible mechanism of Rg1 on Cadmium-induced tesicular damage in rat.Methods:30 wistar rats were divided into 3 groups:the control group

the Cd group and the Cd+Rg1 group.The Cd group rats injected intraperitoneally with 0.5 mg/(kg·d) cadmium for 14 days.The rats in the Cd+Rg1 group were pretreated with 75mg/(kg·d) Rg1 by intragastric administration before injected intraperitoneally with 0.5 mg/(kg·d) cadmium.All animals were killed after 14 days

the sperm count

the movable sperm percentage and the abnormal sperm rate in epididymis were analyzed.The content of MT in testis were detected and the apoptosis of testis genital cells were assessed by TdT-mediated dUTP-biotin nick end labeling(TUNEL)technique.Results:14 days after exposed to Cd caused a significant decrease in the sperm count and the movable sperm percentage.On the other hand

the abnormal sperm rate

the content of MT and the apoptosis percent of genital cells in testis were increased significantly.In the Cd+Rg1 group

the sperm count

the movable sperm percentage and the content of MT were higher than that of Cd group

while the abnormal sperm rate and the apoptosis percent of genital cells in testis were significantly lower than that of Cd group.Conclusion:Rg1 have protective effect on on tesicular damage induced by Cd possibly through upregulating the expression of MT in testis.

关键词

人参皂甙Rg1镉睾丸损伤金属硫蛋白

Keywords

Ginsenoside Rg1cadmiumTesicular damageMetallothionein(MT)

references

Views

161

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

REVERSED PHASE HPLC FOR DETERMINATION OF TRACE AMOUNT OF CADMIUM (Ⅱ), ZINC (Ⅱ), LEAD(Ⅱ), COPPER (Ⅱ) WITH MESO-TETRA PHENYL PORPHYRIN

Effects of cadmium on submandibular gland and the protective effects of testosterone in male rats

The effect of ginsenoside-Rg1 on the expression of Bcl-2 and Bax in rabbit lens epithelial cell induced by oxidative injuries

The study of amyotrophic lateral sclerosis like changes caused by chronic cadmium poisoning in rats

Effect of the cadmium on the expression of vimentin & E-cadherin in sertoli cell and the ultrastructure of blood-testis barrier in rats

Related Author

邱宗荫

李惠芝

LI Xinzhi

et al

He Yongchuan

et al

XIE Wu-tao

et al

Related Institution

重庆医科大学中心实验室

重庆医科大学化学教研室

重庆医科大学中心实验室重庆医科大学化学教研室

Laboratory of Electron Microscopy,Chongqing Medical University

重庆医科大学电子显微镜室生殖医学超微结构实验室

⁰