Subcloning,expressing,purifying and primary studing the biological activity of F3T7

LIU Yang; et al

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Subcloning,expressing,purifying and primary studing the biological activity of F3T7

更新时间：2024-01-17

- Subcloning,expressing,purifying and primary studing the biological activity of F3T7
- Journal of Chongqing Medical University Issue 3, Pages: 239-242(2007)
- 作者机构：
  
  1. 重庆医科大学基础医学院生物化学与分子生物学教研室
  2. 重庆医科大学基础医学院生物化学与分子生物学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.2
- Published：2007，
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[1]刘洋,何爱彬,李甲初,蒋雪,曾昭淳.重组蛋白F3T7的亚克隆、表达、纯化及生物学活性的初步鉴定[J].重庆医科大学学报,2007(03):239-242+327.

LIU Yang, et al. Subcloning,expressing,purifying and primary studing the biological activity of F3T7. [J]. Journal of Chongqing Medical University (3):239-242(2007)
[1]刘洋,何爱彬,李甲初,蒋雪,曾昭淳.重组蛋白F3T7的亚克隆、表达、纯化及生物学活性的初步鉴定[J].重庆医科大学学报,2007(03):239-242+327. DOI：

LIU Yang, et al. Subcloning,expressing,purifying and primary studing the biological activity of F3T7. [J]. Journal of Chongqing Medical University (3):239-242(2007) DOI：

摘要

目的:克隆编码F3T7的DNA序列

获得融合蛋白F3T7

研究其F3T7的功能。方法:采用亚克隆技术获得原核表达载体PET32a（+）-F3TwM法测定F3T7的抗血管活性。结果:F3T7具有特异结合血管内皮细胞能力

并抑制其增殖和新血管生成。结论:初步表明F3T7仍具特异结合血管内皮细胞

抑制新血管生长的能力。

Abstract

Objective:To clone the DNA sequence coding the F3T7

generate the fused protein F3T7

and investigate its function. Methods:We constructed prokaryotic expression vector pET32a(+)-F3T7 by subcloning technique

then fusion protein F3T7 was expressed and purified by affinity chromatography.and its activity of inhibiting the perliferation of HUVEC was assayed by MTT

then FITC was adopted to mark F3T7 to test its affinity with HUVEC

and its anti-angiogenses was detected by CAM. Results:We primarily certified that the fusion protein F3T7 selectively bound to HUVEC

inhibited its proliferation

and showed anti-angiogenic activity.Conclusion:F3T7 could have the abilities of binding to HUVEC and anti-angiogenesis

关键词

亚克隆融合表达抗血管生成

Keywords

SubcloneFusion expressionanti-angiogenesis

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The Department of Biochemistry,Academy of Basic Medicine,Chongqing Medical University

重庆医科大学基础医学院生物化学教研室

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