Simultaneous determination of jasminoidin and paeoniflorin in Qiyitang granules by RP-HPLC

ZHOU Qing; et al

您当前的位置：

首页 >

文章列表页 >

Simultaneous determination of jasminoidin and paeoniflorin in Qiyitang granules by RP-HPLC

更新时间：2024-01-17

- Simultaneous determination of jasminoidin and paeoniflorin in Qiyitang granules by RP-HPLC
- Journal of Chongqing Medical University Issue 3, Pages: 268-270(2007)
- 作者机构：
  
  1. 重庆医科大学药学院药物分析教研室
  2. 贵州遵义医学院附属医院肝胆胰外科
  3. 重庆医科大学药学院药物分析教研室重庆400016 贵州遵义医学院药学系
  4. ,贵州,遵义,563003
- 作者简介：
- 基金信息：
- DOI：
  CLC： R286.0
- Published：2007，
扫描看全文
[1]周卿,兑丹华,代广会,尚京川.RP-HPLC法同时测定清胰汤颗粒中栀子苷、芍药苷的含量[J].重庆医科大学学报,2007(03):268-270.

ZHOU Qing, et al. Simultaneous determination of jasminoidin and paeoniflorin in Qiyitang granules by RP-HPLC. [J]. Journal of Chongqing Medical University (3):268-270(2007)
[1]周卿,兑丹华,代广会,尚京川.RP-HPLC法同时测定清胰汤颗粒中栀子苷、芍药苷的含量[J].重庆医科大学学报,2007(03):268-270. DOI：

ZHOU Qing, et al. Simultaneous determination of jasminoidin and paeoniflorin in Qiyitang granules by RP-HPLC. [J]. Journal of Chongqing Medical University (3):268-270(2007) DOI：

摘要

目的:建立清胰汤颗粒中栀子苷和芍药苷含量的RP-HPLC测定方法。方法:Angilent Eclipse XDB-C18柱（4.5×150mm

5μm）

流动相:乙腈-0.1%磷酸（12∶88

V/V）

流速:1ml/min

检测波长:230nm

柱温:30℃。结果:栀子苷和芍药苷得到完全分离

两种成分线性关系良好。栀子苷高、中、低3个浓度平均加样回收率（n=3）分别为106.2%

97.5%

98.1%

RSD分别为0.96%

1.23%

1.62%;芍药苷高、中、低3个浓度平均加样回收率（n=3）分别为101.3%

99.5%

98.2%

RSD分别为1.19%

1.33%

1.58%。结论:本法操作简便

重复性好

结果准确可靠。

Abstract

Objective:To establish a method for the determination of jasminoidin and paeoniflorin in Qiyitang Granules.Methods:The analytical column was a Agilent Eclipse XDB-C18 column(4.5×150mm

5μm).The mobile phase consisted of acetonitrile-0.1%H3PO4(12:88

V/V)

flow rate was 1.0ml/min.The detection wavelength was 230 nm.Rusults:Jasminoidin and paeoniflorin were separated utterly and the linear correlation was good. The average recoveries of high

middle and low concentration of jasminoidin were 106.2%

97.5% and 98.1% respectively with RSD of 0.96%

1.23% and 1.62%(n=3)respectively.The average recoveries from paeoniflorin were 101.3%

99.5% and 98.2% respectively with RSD being 1.19%

1.33% and 1.58%(n=3)

respectively. Conclusions:The method is simple

reproducible

accurate and reliable.It may be used for the quality control of this granules.

关键词

RP-HPLC清胰汤颗粒栀子苷芍药苷含量测定

Keywords

HPLCQingyitang granulesJasminoidinpaeoniflorinDetermination

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation and Determination of Chondroitin Sulfate

Effects of paeoniflorin on bilirubin-induced apoptosis and NF-κB activity of hippocampal neurons

Effect of paeoniflorin on superoxide dismutase and nuclear factor E2-related factor 2 expression in brain tissues of rats with middle cerebral artery occlusion and its neuroprotection

Related Author

Tang Xianjue

et al

耿娓琴

杨晓兰

ZHU Ye-fang

DANG Shan-shan

TANG Lei

HUA Zi-yu

Related Institution

Research laboratory of pharmaceutical chemistry and biomaterials,college of basic medicalsciences

Department of Neonatology,the Children’s Hospital,Chongqing Medical University

Ministry of Education Key Laboratory of Child Development and Disorder

Medical Biochemistry and Molecular Pharmacology Laboratory，School of Pharmacy，Chongqing Medical University

⁰