Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa

REN Qinglan; et al

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Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa

更新时间：2024-01-17

- Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa
- Journal of Chongqing Medical University Issue 9, Pages: 946-950(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 重庆市肿瘤医院放疗科
  3. 重庆医科大学基础医学院核医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2007，
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[1]任庆兰,吴永忠,陈晓品,李少林.抑制c-raf-1基因对人宫颈癌细胞放射敏感性影响的实验研究[J].重庆医科大学学报,2007(09):946-950.

REN Qinglan, et al. Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa. [J]. Journal of Chongqing Medical University (9):946-950(2007)
[1]任庆兰,吴永忠,陈晓品,李少林.抑制c-raf-1基因对人宫颈癌细胞放射敏感性影响的实验研究[J].重庆医科大学学报,2007(09):946-950. DOI：

REN Qinglan, et al. Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa. [J]. Journal of Chongqing Medical University (9):946-950(2007) DOI：

摘要

目的:探讨用反义技术抑制癌基因c-raf-1对宫颈癌细胞（HeLa）放射敏感性的影响。方法:设立空白对照组、c-raf-1正义寡核苷酸组和脂质体组（仅加入等量的脂质体）作对照

用RT-PCR检测宫颈癌细胞处理前后c-raf-1表达水平的变化。用集落形成试验检测各实验组照射后人宫颈癌HeLa细胞株集落形成率

AO/EB荧光染色法检测细胞凋亡率的变化

流式细胞仪测定Bcl-2蛋白的表达。结果:c-raf-1反义寡核苷酸能有效抑制c-raf-1癌基因的表达

经脂质体介导的c-raf-1反义寡核苷酸作用的宫颈癌细胞经60Coγ射线照射后其集落形成率较正义寡核苷酸组及单纯照射组明显下降

而凋亡率明显增加

Bcl-2蛋白的表达显著下调

与对照各组比较有显著差异（P<0.01）。结论:c-raf-1反义寡核苷酸通过抑制c-raf-1基因降低人宫颈癌HeLa细胞放射抗性。其辐射增敏作用可能与c-raf-1反义寡核苷酸抑制了辐射抵抗信号转导途径

增加照射后肿瘤细胞的凋

Abstract

Objective:To explore the effect of inhibiting c-raf-1 by antisense technology on radiosensitivity in human cervical carcinoma cell line HeLa.Methods:There were four groups in the study:control group

lipofectin group

sense oligodexynucleotides(S-ODN) group

antisense oligodexynucleotides(AS-ODN) group.The expression of c-raf-1 was detected by means of RT-PCR

Cellular response to irradiation was evaluated by the colony forming test

Apoptosis was observed by fluorescent staining.Bcl-2 protein expression was determined by flow cytometry.Results:AS-ODN group could significantly decreased the proliferation rate and increasing the apoptosis rate

significantly downregulating the expression of Bcl-2 of irradiated human cervical carcinoma cells

(vs lipofectin group or S-ODN group

P<0.01).Conclusion:c-raf-1 antisense oligodexynucleotides sensitized the HeLa to ionizing irradiation through decreasing the expression of c-raf-1

which might be related to inhibiting the celluar radiation-resistant signal transduction pathway and increasing the apoptosis rate of HeLa cells.

关键词

人宫颈癌细胞株HeLac-raf-1反义技术放射敏感性

Keywords

Human cervical carcinoma cell line HeLac-raf-1Antisense technologyradiosensitivity

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