Effect of morphine dependence and abstinence on the expression of B-cell Lymphomal-2 in Sprague-Dawley rats

YU Huiping; et al

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Effect of morphine dependence and abstinence on the expression of B-cell Lymphomal-2 in Sprague-Dawley rats

更新时间：2024-01-17

- Effect of morphine dependence and abstinence on the expression of B-cell Lymphomal-2 in Sprague-Dawley rats
- Journal of Chongqing Medical University Issue 8, Pages: 799-801(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院心理卫生中心
  2. 重庆医科大学附属第一医院心理卫生中心重庆40016乐山市人民医院神经内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R749.6
- Published：2007，
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[1]余会平,蒙华庆,胡华,罗庆华,邓伟,傅一笑.吗啡依赖及戒断大鼠相关脑区Bcl-2的表达研究[J].重庆医科大学学报,2007(08):799-801+811.

YU Huiping, et al. Effect of morphine dependence and abstinence on the expression of B-cell Lymphomal-2 in Sprague-Dawley rats. [J]. Journal of Chongqing Medical University (8):799-801(2007)
[1]余会平,蒙华庆,胡华,罗庆华,邓伟,傅一笑.吗啡依赖及戒断大鼠相关脑区Bcl-2的表达研究[J].重庆医科大学学报,2007(08):799-801+811. DOI：

YU Huiping, et al. Effect of morphine dependence and abstinence on the expression of B-cell Lymphomal-2 in Sprague-Dawley rats. [J]. Journal of Chongqing Medical University (8):799-801(2007) DOI：

摘要

目的:明确吗啡依赖及戒断两种状态时大鼠相关脑区Bcl-2的表达特征。方法:将18只Sprague-Dawley雄性大鼠随机分为吗啡依赖组、吗啡戒断组和空白对照组

每组6只。采用递增法皮下注射吗啡建立大鼠吗啡依赖模型

纳络酮注射戒断

应用免疫组化和地高辛标记寡核苷酸探针原位杂交技术检测三组大鼠不同脑区Bcl-2的表达水平。结果:连续给予吗啡5天及纳络酮戒断后

中脑腹侧被盖区、海马、蓝斑、前额叶皮质脑区Bcl-2及其mRNA较对照组显著降低（P<0.05）

而吗啡戒断组伏隔核脑区内Bcl-2及其mRNA的表达较依赖组显著增加（P<0.05）

结论:吗啡依赖及戒断大鼠Bcl-2蛋白及其mRNA的表达在不同脑区有选择性的改变

可能是吗啡依赖及戒断的分子机制之一。

Abstract

Objective:To investigate the expression of Bcl-2 in rat brain during dependence and withdral state.Methods:The rats were rendered dependent by subcutaneous injections of increasing doses of morphine from 10 to 80 mg/kg for 5 consecutive days.The rats as controls were administered with same volume of normal saline.The expression of Bcl-2 mRNA was assayed with digacin-labelled Bcl-2 cDNA probe by in situ hybridization and the levels of Bcl-2 protein were measured by immunohistochemistry.Results:The evels of Bcl-2 and their mRNA in prefrontal cortex

locus coeruleus

hippocampus and ventral tegmental area significantly decreased as comparied with that in control rats after chronic administration of morphine and naloxone administration.However

Bcl-2 and their mRNA in nucleus accumbens of rats in withdral state were markedly higher than that in nucleus accumbens of morphine dependent rats.Conclusion:Chronic exposure to morphine appears to induce modification of the expression of Bcl-2 mRNA and their protein of rats in the rat brain

which contributes to the development of morphine dependence.

关键词

吗啡依赖戒断综合征Bcl-2

Keywords

Morphine dependenceSubstance withdrawal syndromeBcl-2

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