The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes

CHANG Jing; et al

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The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes

更新时间：2024-01-17

- The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes
- Journal of Chongqing Medical University Issue 9, Pages: 907-910(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院心内科
  2. 重庆医科大学附属第一医院血液内科
  3. 垫江县妇幼保健院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R542.22
- Published：2007，
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[1]常静,雷寒,陈建斌,黄仁英.大鼠心肌细胞诱导人骨髓间充质干细胞向心肌样细胞分化的研究[J].重庆医科大学学报,2007(09):907-910.

CHANG Jing, et al. The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes. [J]. Journal of Chongqing Medical University (9):907-910(2007)
[1]常静,雷寒,陈建斌,黄仁英.大鼠心肌细胞诱导人骨髓间充质干细胞向心肌样细胞分化的研究[J].重庆医科大学学报,2007(09):907-910. DOI：

CHANG Jing, et al. The study about the cardiac differentiation of human mesenchymal stem cells induced by rat cardiomyocytes. [J]. Journal of Chongqing Medical University (9):907-910(2007) DOI：

摘要

目的:研究人间充质干细胞（MSCs）向心肌方向分化的机制。方法:将人MSCs以10000/㎝2的密度接种

共分为四组:1)对照组;2)共同培养组:人MSCs和新生SD大鼠心肌细胞进行共同培养;3)条件培养液组:将从新生SD大鼠心肌细胞培养中获得的培养液

用于人MSCs的培养;4)细胞匀浆液组:将心肌细胞匀浆液用于人MSCs的培养。分别于实验第2、5、7天收集各实验组的人MSCs

进行Desmin及肌钙蛋白I的免疫组化染色。在共同培养组中

同时还用抗人DNA进行的荧光原位杂交

以区分鼠来源的细胞及人来源的细胞。结果:在对照组、条件培养液组及细胞匀浆液组均未检测到心肌特异性蛋白的表达。在共培养组中

第2天

部分人MSCs开始表达Desmin

第5天细胞开始表达cTnI

至第7天阳性表达率达35%左右。结论:与大鼠心肌细胞共同培养可以诱导人MSCs向心肌方向的分化

而心肌细胞的条件培养液及心肌细胞匀浆不能诱导它们的心肌分化。

Abstract

Objective: To explore the mechanism about the cardiac differentiation of human mesenchymal stem cells(MSCs) induced by rat cardiomyocytes.Methods:To induce the cardiac differentiation of human MSCs by using rat cardiomyocytes in different ways(three dishes for each way):(1) Cocultured with rat cardiomyocytes at the ratio of 1:1;(2)Cultured with the conditional medium

which got from the culture of rat cardiomyocytes and mixed with fresh medium by 1:1;(3) Cultured with the supernatant

which got from the minced rat cardiomyocytes and mixed with fresh medium by 1:10.The human MSCs were harvested at the day of 2

5 and 7

respectively.Then the cells were stained with desmin and cardiac troponin I monoclonal-antibodies

and fluorescent in situ hybridization to human DNA was used to mark the cells from human.Results: No desmin or cTnI was detected in the human MSCs cultured with conditional medium or supernatant.While for the human MSCs cocultured with rat cardiomyctes

desmin was detectable at the day of 2 and cTnI at the day of 5.Until the day of 7

about 35% of cocultured-human MSCs expressed those cardiac specific antigens.Conclusion:Human MSCs could be induced to differentiate into cardiomyocyte-like cells by coculture with rat cardiomyocytes

but not by the conditional medium or the supernatant of rat cardiomyocytes.

关键词

间充质干细胞心肌细胞共同培养心肌分化

Keywords

mesenchymal stem cellscardiomyocytesCocultureCardiac differentiation

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