Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats

RAN Jianhua; et al

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Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats

更新时间：2024-01-17

- Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats
- Journal of Chongqing Medical University Issue 9, Pages: 901-906(2007)
- 作者机构：
  
  1. 重庆医科大学基础医学院解剖学教研室神经生物研究室
  2. 重庆医科大学应用技术学院附属医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R743
- Published：2007，
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[1]冉建华,孙善全,汪克建,毕恣,陈海,杨美.水通道蛋白9表达与大鼠缺血性脑水肿形成的关系[J].重庆医科大学学报,2007(09):901-906+950.

RAN Jianhua, et al. Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats. [J]. Journal of Chongqing Medical University (9):901-906(2007)
[1]冉建华,孙善全,汪克建,毕恣,陈海,杨美.水通道蛋白9表达与大鼠缺血性脑水肿形成的关系[J].重庆医科大学学报,2007(09):901-906+950. DOI：

RAN Jianhua, et al. Relationship between AQP9 expression and formation of ischemic cerebral edema in the rats. [J]. Journal of Chongqing Medical University (9):901-906(2007) DOI：

摘要

目的:研究水通道蛋白9（Aquaporin9

AQP9）在大鼠缺血性脑组织中的表达及其与脑水肿形成之间的关系。方法:采用线拴法制作大鼠局灶性脑缺血模型

通过光镜、电镜技术观察脑水肿区的病理变化

干湿重法检测脑含水量

采用原位杂交、免疫组化方法观察AQP9及其mRNA在脑组织中的表达。结果:缺血后细胞及血管周围间隙明显扩大

部分细胞坏死

胶质细胞增生;大鼠穹隆下器官、脉络丛、大脑皮质、视上核、海马等部位AQP9及其mRNA的表达上调

48￣72h达峰值;脑组织含水量亦在72h达高峰。结论:随缺血性脑水肿的出现

AQP9及其mRNA表达上调

提示AQP9可能在缺血性脑水肿的形成中发挥重要作用。

Abstract

Objective:To study the role of AQP9 and its mRNA expression in the formation of ischemic cerebral edema in the rats.Methods:MCAO models were established by occluding unilateral middle cerebral artery(MCA) of the rats with the suture method.The morphological changes were observed with HE stain and transmission electron microscope(TEM).Brain water content(BWC) was measured by using wet-dry weighing method.The expression of AQP9 and its mRNA in the edema brain tissue were detected with in situ hybrilization(ISH) and immunohistochemistry(IHC).Results:The space around the neurons and perivascular space became larger.Some neurons showed necrosis phenotype

and the number of the neurogliocyte increased.The expression of AQP9 and its mRNA were up-regulated in subfornical organ(SFO)、choroid plexes、brain cortex、supraoptic nuclei、hippocampus and other regions

and reached its peak at 48~72h after MCAO.The brain water content(BWC) in ischemic cerebral tissue reached its peak at 72h.Conclusions:the expression of AQP9 and its mRNA were up-regulated following the brain edema.The results imply that AQP9 might play an important role in the formation of ischemic cerebral edema.

关键词

AQP9大鼠缺血性脑水肿

Keywords

AQP9ratIschemic cerebral edema

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