Experimental study on the effects of Mad1 to bladder tumor in vivo

HU Hongbo; et al

您当前的位置：

首页 >

文章列表页 >

Experimental study on the effects of Mad1 to bladder tumor in vivo

更新时间：2024-01-17

- Experimental study on the effects of Mad1 to bladder tumor in vivo
- Journal of Chongqing Medical University Issue 9, Pages: 919-921(2007)
- 作者机构：
  
  1. 重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室、重庆重点实验室
  2. 重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.14
- Published：2007，
扫描看全文
[1]胡宏波,罗春丽,唐敏,蔡晓钟,赵培,吴小候.Mad1对膀胱肿瘤作用的体内实验研究[J].重庆医科大学学报,2007(09):919-921+958.

HU Hongbo, et al. Experimental study on the effects of Mad1 to bladder tumor in vivo. [J]. Journal of Chongqing Medical University (9):919-921(2007)
[1]胡宏波,罗春丽,唐敏,蔡晓钟,赵培,吴小候.Mad1对膀胱肿瘤作用的体内实验研究[J].重庆医科大学学报,2007(09):919-921+958. DOI：

HU Hongbo, et al. Experimental study on the effects of Mad1 to bladder tumor in vivo. [J]. Journal of Chongqing Medical University (9):919-921(2007) DOI：

摘要

目的:在原位建立膀胱肿瘤的大鼠模型

观察转染Mad1表达质粒后对膀胱肿瘤增殖的抑制作用。方法:MNU对SD大鼠进行膀胱灌注诱发膀胱肿瘤

荷瘤动物随机分为3组:Mad1转染组（A组）

空载体转染组（B组）和生理盐水转染组（C组）。通过大鼠重量（RBW）、膀胱绝对重量（BAW）、膀胱相对重量（BRW）、Mad1和TERT mRNA表达水平等指标

用流式细胞仪等检测手段

比较转染前后Mad1和TERT的表达差异、观察Mad1对膀胱肿瘤增殖的抑制作用以及探讨相应的机理。结果:成功在原位建立膀胱肿瘤的大鼠模型。A组与B、C两组相比RBW无显著性差异（P>0.05）

但BAW和BRW分别有非常显著差异性（P<0.01）及显著差异性（P<0.05）;Mad1mRNA相对表达量明显增加;TERT mRNA相对表达量明显降低;流式细胞仪显示G0/G1期细胞显著增加（P<0.01）

S期细胞显著减少（P<0.01）.结论:高表达的Mad1能抑制膀胱肿瘤的增殖。

Abstract

Objective:To construct the orthotopic model of bladder tumor in rats and to observe the inhibition effect on bladder tumor after transfected with expression plasmid pcDNA3.1(+)/Mad1.Methods:Bladder tumors were induced in SD rats by intravascular instillation of MNU.The rats bearing tumor were randomly divided into 3 groups:transfected with pcDNA3.1(+)/Mad1(group A)

transfected with empty vector(group B) and transfected with saline(group C).Tumor-bearing rats were transfected using Lipofectamine.Rat body weight(RBW)

Bladder absolute weight(BAW)

bladder relative weight(BRW) and expression levels of mRNA of Mad1 and TERT were assayed and flow cytometer analysis were used to observe the inhibition effect of Mad1 to bladder tumor.Results:Compared with group B and group C

in group A

though there was no diference in RBW(P>0.05)

BAW and BRW were significantly decreased(P<0.01 and P<0.05

respectively).Mad1 mRNA expression levels were markedly improved

while TERT mRNA expression levels were markedly decreased.Flow cytometry showed an increase of G0/G1-phase cells and a decrease of S-phase cells after transfected with Mad1.Conclusions:Over expression of Mad1 can inhibit the proliferation of bladder tumor.

关键词

膀胱肿瘤动物模型细胞周期转染

Keywords

Bladder tumoranimal modelcell cycletransfection

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Promotion of the proliferation of hepatocellular carcinoma cells by acetyl-CoA carboxylase 2 through cell cycle regulation

A mouse model of polycystic ovary syndrome with abnormal glucose and lipid metabolism induced by dihydrotestosterone combined with high-fat diet

Inhibitory effect of SAHA on human osteosarcoma 143B cells and its related mechanism

Etablishment and evaluation standards of skin photoaging model in SD rat

Erythropoietin promotes the proliferation of glioblastoma cells through up-regulation of Cyclin D1 in vitro

Related Author

Zhang Zhirong

Wang Kai

Tang Ni

Wu Jinlin

Zhang Binghan

Long Jiangchuan

Fang Ronghua

Chen Jie

Related Institution

Key Laboratory of Molecular Biology of Infectious Diseases Designated by the Chinese Ministry of Education， Chongqing Medical University

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

Department of Endocrinology，The Second Affiliated Hospital of Chongqing Medical University

Key Laboratory of Medical Diagnostics Founded by Ministry of Education,College of Laboratory Medicine,Chongqing Medical University

College of Lab Medicine,Hebei North University

⁰