Study on effect of celecoxib on CNE-2Z cell lines in human nasopharyngeal carcinoma

XIANG Yinzhou; et al

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Study on effect of celecoxib on CNE-2Z cell lines in human nasopharyngeal carcinoma

更新时间：2024-01-17

- Study on effect of celecoxib on CNE-2Z cell lines in human nasopharyngeal carcinoma
- Journal of Chongqing Medical University Issue 11, Pages: 1154-1157(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学附属第一医院耳鼻咽喉科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.63
- Published：2007，
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[1]向银洲,魏莲枝,李洁.塞来昔布对鼻咽癌细胞株CNE-2Z作用的研究[J].重庆医科大学学报,2007(11):1154-1157+1161.

XIANG Yinzhou, et al. Study on effect of celecoxib on CNE-2Z cell lines in human nasopharyngeal carcinoma. [J]. Journal of Chongqing Medical University (11):1154-1157(2007)
[1]向银洲,魏莲枝,李洁.塞来昔布对鼻咽癌细胞株CNE-2Z作用的研究[J].重庆医科大学学报,2007(11):1154-1157+1161. DOI：

XIANG Yinzhou, et al. Study on effect of celecoxib on CNE-2Z cell lines in human nasopharyngeal carcinoma. [J]. Journal of Chongqing Medical University (11):1154-1157(2007) DOI：

摘要

目的:探讨塞来昔布对鼻咽癌细胞株CNE-2Z的生长抑制作用及可能机制。方法:以人鼻咽癌细胞株CNE-2Z为研究对象

体外药物敏感试验（MTT）观察不同浓度和作用时间的塞来昔布对细胞的生长抑制作用;免疫细胞化学染色检测细胞增殖核抗原（PCNA）和环氧化酶-2（COX-2）的表达;流式细胞术（FCM）检测药物作用后细胞周期分布。结果:MTT试验显示塞来昔布对人鼻咽癌细胞株CNE-2Z增殖有抑制作用

其抑制效应具有时间及浓度依赖的特点;流式细胞术表明塞来昔布可引起CNE-2Z细胞G0/G1期阻滞;免疫组织化学检测显示塞来昔布下调PCNA、COX-2蛋白表达。结论:CNE-2Z细胞中存在COX-2表达

塞来昔布可抑制鼻咽癌CNE-2Z细胞增殖

可能成为鼻咽癌化疗的有效药物。

Abstract

Objective:To study the growth inhibition and potential mechanisms of celecoxib on nasopharyngeal carcinoma CNE-2Z cell lines.Methods:The effect of celecoxib on the growth of CNE-2Z cells was evaluated by MTT assay.The expressions of proliferating cell nuclear antigen(PCNA)and cyclooxygenase-2(COX-2)were assessed by immunocytochemistry method.Cell cycle was measured by Flow cytometry(FCM).Results:Celecoxib inhibited the proliferation of CNE-2Z cells in dosage and time dependent manner.The cell cycle was arrested in G0/G1 phase in CNE-2Z cells treated with celecoxib;Immunocytochemistry showed that the expressions of PCNA and C0X-2 decreased after incubated with celecoxib for 48 hours.Conclusion:The expression of COX-2 is found in CNE-2Z cell lines.The growth of CNE-2Z cell 1ines can be inhibited by celecoxib.Celecoxib may be used as chemotherapeutic agent for nasopharyngeal carcinoma.

关键词

塞来昔布鼻咽癌细胞株CNE-2Z生长抑制

Keywords

celecoxibCell lines in nasopharyngeal carcinomaGrowth inhibition.

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