Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide

FU Yueqiang; et al

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Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide

更新时间：2024-01-17

- Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide
- Journal of Chongqing Medical University Issue 11, Pages: 1150-1153(2007)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院急诊科
  2. 重庆医科大学附属儿童医院急诊科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R720.597
- Published：2007，
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[1]符跃强,许峰,卢仲毅,匡凤梧,王兴勇.氧化应激对肺泡Ⅱ型上皮细胞的损伤作用及JNK信号转导机制[J].重庆医科大学学报,2007(11):1150-1153.

FU Yueqiang, et al. Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide. [J]. Journal of Chongqing Medical University (11):1150-1153(2007)
[1]符跃强,许峰,卢仲毅,匡凤梧,王兴勇.氧化应激对肺泡Ⅱ型上皮细胞的损伤作用及JNK信号转导机制[J].重庆医科大学学报,2007(11):1150-1153. DOI：

FU Yueqiang, et al. Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide. [J]. Journal of Chongqing Medical University (11):1150-1153(2007) DOI：

摘要

目的:探讨氧化应激状态下肺泡II型上皮细胞（Alveolar type Ⅱ epithelial cell

ATⅡ）存活、凋亡和JNK（c-jun NH2-terminal kinase）的调控机制。方法:采用过氧化氢（Hydrogen peroxide

H2O2）刺激原代大鼠ATⅡ细胞

复制活性氧（Reactive oxygen species

ROS）攻击ATⅡ细胞损伤模型。蛋白质免疫印迹法（Western blot）检测ATⅡ细胞受500μmol/LH2O2刺激后磷酸化JNK的动态变化

四甲基偶氮唑盐反应比色法（MTT法）检测细胞存活率

流式细胞术检测细胞凋亡率

并观察JNK抑制剂SP600125干预前后存活率和凋亡率的变化。结果:ATⅡ细胞在受H2O2刺激后随作用时间的延长细胞存活率下降

凋亡率增加;H2O2刺激可导致JNK的磷酸化激活

使用SP600125后

细胞存活率增加

凋亡率降低。结论:H2O2以时间依赖的方式诱导ATⅡ细胞凋亡

抑制JNK信号激活对氧化应激状态下的ATⅡ细胞可能起到保护作用。

Abstract

Objective:To investigate survival and apoptotic responses of alveolar epithelial typeⅡcells(ATⅡ cell)under oxidative stress and to discuss the regulation mechanism mediated by c-jun NH2-terminal kinase(JNK).Methods:Primary rat alveolar epithelial typeⅡcells were attacked by Hydrogen peroxide(H2O2)and cells were pretreated with SP600125 in some groups.Cell viability

apoptotic rate and the expression of phosphorylated JNK(p-JNK)were measured by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide(MTT)assay

flow cytometry and western blot analysis

respectively.Results:Compared with control group

decreased cell viability and increased apoptotic rate in ATⅡ cells occurred in time-dependent manner when treated with 500μmol/L H2O2 in different time.H2O2 induces phosphorylation of JNK.SP600125 enhanced cell viability and decreased apoptotic rate after H2O2-exposure.Conclusion:Apoptosis could be induced by H2O2 in ATⅡ cells in time-dependent manner.JNK signaling pathway may play a proapoptotic role in the regulation of apoptosis induced by H2O2.

关键词

肺泡Ⅱ型上皮细胞凋亡c-Jun氨基端激酶活性氧

Keywords

Alveolar epithelial type Ⅱ cellapoptosisc-jun NH2-terminal kinasereactive oxygen species

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