Empirical study on ursolic acid inducing apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z

LI Jie; et al

您当前的位置：

首页 >

文章列表页 >

Empirical study on ursolic acid inducing apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z

更新时间：2024-01-17

- Empirical study on ursolic acid inducing apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z
- Journal of Chongqing Medical University Issue 1, Pages: 37-40(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学附属第一医院耳鼻咽喉科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.63
- Published：2008，
扫描看全文
[1]李洁,魏莲枝,向银洲.熊果酸诱导鼻咽癌细胞株CNE-2Z凋亡的实验研究[J].重庆医科大学学报,2008(01):37-40.

LI Jie, et al. Empirical study on ursolic acid inducing apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z. [J]. Journal of Chongqing Medical University (1):37-40(2008)
[1]李洁,魏莲枝,向银洲.熊果酸诱导鼻咽癌细胞株CNE-2Z凋亡的实验研究[J].重庆医科大学学报,2008(01):37-40. DOI：

LI Jie, et al. Empirical study on ursolic acid inducing apoptosis in human nasopharyngeal carcinoma cell line CNE-2Z. [J]. Journal of Chongqing Medical University (1):37-40(2008) DOI：

摘要

目的:探讨熊果酸诱导人鼻咽癌细胞CNE-2Z凋亡的作用机制。方法:用不同浓度的熊果酸处理CNE-2Z细胞

采用四甲基偶氮唑蓝（MTT）比色检测熊果酸对CNE-2Z细胞的增殖抑制效应;流式细胞术分析细胞周期和凋亡率;应用免疫组织化学SP法检测凋亡相关基因bcl-2

bax和cox-2表达的变化;Western blot检测CNE-2Z细胞内caspase 3的表达。结果:熊果酸对CNE-2Z细胞具有抑制增殖和诱导凋亡作用

并呈浓度依赖性;流式细胞术分析表明S期细胞数减少

细胞主要阻滞在G0/G1期;免疫组织化学检测显示在凋亡过程中bax表达上调

bcl-2、cox-2表达下调;Western blot检测CNE-2Z细胞中caspase 3表达增强。结论:熊果酸在体外对CNE-2Z细胞有诱导凋亡的作用

其作用机制可能是通过促进caspase 3的活化和bax的表达上调、bcl-2、cox-2的表达下调来实现。

Abstract

Objective:To investigate the mechanism of ursolic acid inducing the apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z.Methods:CNE-2Z were treated by ursolic acid with different concentration.Cell proliferation was determined by MTT assay.Cell cycle and apoptosis rate were analysed by flow cytometry(FCM).The expressions of bax

bcl-2 and cox-2 were assessed by SP method of immunocytochemistry

while the expression of caspase 3 by Western blot.Results:Ursolic acid inhibited the proliferation of CNE-2Z cells and induced the apoptosis in dosage and time dependent manner.FCM showed that the cell population reduced in S phase and cell cycle was arrested in G0/G1 phase.Immunocytochemistry showed that the expression of bax was up-regulated in the apoptosis

while the expression of bcl-2 and cox-2 down-regulated.Western blot showed that the expression of caspase 3 was strengthened.Conclusion:Ursolic acid can induce apoptosis of CNE-2Z in vitro.Its molecular mechanism probably is through promoting activation of caspase3

up-regulating the expression of bax

and down-regulating the expressions of bcl-2 and cox-2.

关键词

熊果酸鼻咽癌细胞CNE-2Z凋亡

Keywords

ursolic acidNasopharyngeal carcinoma cellCNE-2Zapoptosis

references

Views

139

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Empirical study on ursolic acid inducing apoptosis in human prostatic carcinoma cell line PC-3

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

TNF-α downregulates the expression of tight junction proteins ZO-1 and Claudin-5 in rat pulmonary microvascular endothelial cells

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

XL413 inhibits colon cancer cell proliferation by inhibiting DNA replication and inducing cell apoptosis

Related Author

XIA Yang

et al

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Bai Jijia

Shi Guocui

Related Institution

Department of Urology,the First Affiliated Hospital,Chongqing Medical University

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Critical Care Medicine,General Hospital of Ningxia Medical University

Department of Respiratory Medicine,Cangzhou People's Hospital

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

⁰