Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells

WEN Xian-hao; et al

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Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells

更新时间：2024-01-17

- Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells
- Journal of Chongqing Medical University Issue 1, Pages: 41-44(2008)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院血液科
  2. 重庆医科大学附属儿童医院血液科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R733.7
- Published：2008，
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[1]温贤浩,徐酉华.survivin反义寡核苷酸对K562细胞增殖的抑制作用[J].重庆医科大学学报,2008(01):41-44+48.

WEN Xian-hao, et al. Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells. [J]. Journal of Chongqing Medical University (1):41-44(2008)
[1]温贤浩,徐酉华.survivin反义寡核苷酸对K562细胞增殖的抑制作用[J].重庆医科大学学报,2008(01):41-44+48. DOI：

WEN Xian-hao, et al. Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells. [J]. Journal of Chongqing Medical University (1):41-44(2008) DOI：

摘要

目的:采用反义寡核苷酸技术研究凋亡抑制基因survivin对白血病细胞增殖的影响。方法:1.细胞抑制率实验分5组:111nmol/L反义寡核苷酸组、333nmol/L反义寡核苷酸组、555nmol/L反义寡核苷酸组、777nmol/L反义寡核苷酸组、空白组。转染后24、48、72h收集细胞进行检测:用台盼蓝染色进行活细胞计数

计算细胞抑制率。2.细胞周期及survivin蛋白表达实验分4组:555 nmol/L寡核苷酸组、544nmol/L无义组、脂质体组、空白组

转染后24、48h流式细胞仪检测细胞周期;免疫组织化学方法检测survivin蛋白水平。结果:不同浓度的反义寡核苷酸对K562细胞的细胞抑制率均高于空白组（P<0.05）

并随反义寡核苷酸的浓度增大抑制率增加;随作用时间延长

抑制作用越明显

抑制率与浓度及时间呈正相关;555nmol/L反义寡核苷酸作用后

K562细胞被阻滞在G0/G1期

细胞周期进程受到明显阻滞

而无义、脂质体、空白组细胞周期进程无明显变化（P<0.05）;反义寡核苷酸作用48h后survivin蛋白表达水平明显降低

与各对照组比较有显著性差异（P<0.05）。结论:survivin反义寡核苷酸能抑制K562细胞的增殖。

Abstract

Objective:To study the effect of survivin

an inhibitor of apoptosis

on the proliferation of leukemic cell by antisense oligonucleotide(ASON) technique.Methods:1.K562 cells were divided into 5 groups for cell inhibition rate experiment

111nmol/L ASON group

333nmol/L ASON group

555nmol/L ASON group

777nmol/L ASON group and the blank control group.At 24h

48h and 72h after transfection cells were collected to be stained by tapan-blue

and than the number of live cells and the rate of inhibition were counted.2.K562 cells were divided into 4 groups for the cell cycle and survivin protein expression

555nmol/L ASON group

544nmol/L NSON group

lipofectin group and the blank control group.At 24h

48h after transfection

the cell cycle progression was detected by flow cytometry

and the expression of survivin was examined by immunohistochemistry.Results:The rate of inhibition in ASON groups with different concentration was significantly higher than that of the control group

and the rate of inhibition was positively related to concentration of ASON and time of transfection.In 555nmol/L ASON group

the cell number in G0/G1 phase increased

and the cell cycle progression was inhibited significantly;while cell cycle progression in NSON group

lipofectin group and the control group had no significant change(P<0.05).After transfection for 48h

the protein level of survivin in ASON group decreased significantly compared to the other groups(P<0.05).Conclusions:ASON targeting survivin can inhibit proliferation of K562 cells.

关键词

K562细胞反义寡核苷酸细胞增殖Survivin

Keywords

K562 cellsAntisense oligonucleotidecell proliferationSurvivin

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Related Institution

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