Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer

LIU Fang-xin; et al

您当前的位置：

首页 >

文章列表页 >

Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer

更新时间：2024-01-17

- Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer
- Journal of Chongqing Medical University Issue 1, Pages: 49-52(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院放射医学教研室
  2. 重庆医科大学基础医学院放射医学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2008，
扫描看全文
[1]刘方欣,李晓愚,任庆兰,李少林.COX-2抑制剂对乳腺癌放射增敏作用的实验研究[J].重庆医科大学学报,2008(01):49-52.

LIU Fang-xin, et al. Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer. [J]. Journal of Chongqing Medical University (1):49-52(2008)
[1]刘方欣,李晓愚,任庆兰,李少林.COX-2抑制剂对乳腺癌放射增敏作用的实验研究[J].重庆医科大学学报,2008(01):49-52. DOI：

LIU Fang-xin, et al. Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer. [J]. Journal of Chongqing Medical University (1):49-52(2008) DOI：

摘要

目的:探讨环氧化酶2抑制剂尼美舒利对乳腺癌细胞株MCF-7的放射增敏作用并初步探讨其机制。方法:克隆形成实验检测尼美舒利对MCF-7的放射增敏作用;电镜观察肿瘤细胞形态学改变;流式细胞仪（FCM）检测肿瘤细胞调亡率及Bcl-2、Bax蛋白的表达。结果:不同浓度的尼美舒利对乳腺癌细胞MCF-7均有放射增敏作用

且呈剂量依赖性关系;尼美舒利与射线照射联用能够上调Bax的蛋白表达

同时抑制Bcl-2蛋白表达

明显增加细胞凋亡率。结论:COX-2抑制剂尼美舒利对人乳腺癌MCF-7细胞株具有明显的放疗增敏作用。

Abstract

Objective:To investigate the radiosensitivity enhancement effect of COX-2 inhibitor-Nimesulide on breast cancer cell line(MCF-7) in vitro and to initially disclosure its mechanism.Methods:The clonogenic assay was performed to determine the radiosensitizing effect of Nimesulide on MCF-7 cell line.The morphological changes of MCF-7 were observed by electron microscopy.The apoptosis and expressions of Bcl-2 and Bax were measured by flow cytometry(FCM).Results: Nimesulide at different concentration significantly enhanced the radiosensitivity of MCF-7 cells.in a dose-dependent manner

under the combined treatment of Nimesulide and radial the protein expression of Bax was up-regulated

while that of Bcl-2 was inhibited and the apoptpsis increased significantly.Conclusion:Cox-2 inhibitor could obviously enhance the radiosentivity of human breast cancer cell line MCF-7.

关键词

环氧化酶2选择性抑制剂放射敏感性乳腺癌凋亡

Keywords

Selective cyclooxygene-2 inhibitorradiosensitivitybreast cancerapoptosis

references

Views

122

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line

The comparative study of growth inhibition effect of arsenic trioxide on human breast cancer MCF-7 and MCF-7/ADR cell

The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

TNF-α downregulates the expression of tight junction proteins ZO-1 and Claudin-5 in rat pulmonary microvascular endothelial cells

Related Author

SONG Lin

et al

刘芳欣

王树斌

柏玮

彭志平

YIN Guobing

et al

Related Institution

Department of Radiation, College of Basic Medicine,Chongqing Medical University

Department of General Surgery,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院普外科

the Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Chongqing Medical University

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

⁰