Genotyping of 238 HBV strains by the methods of type-specific primers PCR

ZENG Ai-zhong; et al

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Genotyping of 238 HBV strains by the methods of type-specific primers PCR

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- Genotyping of 238 HBV strains by the methods of type-specific primers PCR
- Journal of Chongqing Medical University Issue 1, Pages: 53-56(2008)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所教育部感染性疾病分子生物学重点实验室
  2. 重庆医科大学附属第一医院感染科
  3. 重庆医科大学病毒性肝炎研究所教育部感染性疾病分子生物学重点实验室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R450
- Published：2008，
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[1]曾爱中,黄爱龙,郭进军,黄文祥,李青岭,张祯祯,郝美君.型特异引物PCR法分析238株乙肝病毒基因型[J].重庆医科大学学报,2008(01):53-56.

ZENG Ai-zhong, et al. Genotyping of 238 HBV strains by the methods of type-specific primers PCR. [J]. Journal of Chongqing Medical University (1):53-56(2008)
[1]曾爱中,黄爱龙,郭进军,黄文祥,李青岭,张祯祯,郝美君.型特异引物PCR法分析238株乙肝病毒基因型[J].重庆医科大学学报,2008(01):53-56. DOI：

ZENG Ai-zhong, et al. Genotyping of 238 HBV strains by the methods of type-specific primers PCR. [J]. Journal of Chongqing Medical University (1):53-56(2008) DOI：

摘要

目的:研究乙肝病毒（HBV）感染者HBV基因型分布状况

并建立一套HBV病毒株分型的简便可靠的分析法。方法:设计型特异引物

采用基因型特异引物聚合酶链反应（PCR）法分型。结果:238株HBV中218株得到分型。其中B型149例（男115例、女34例）;C型63例（男47例、女16例）;B+C混合型6例（男4例、女2例）。B型、C型和B+C混合型检出率分别为68.3%（男69.3%、女65.3%）、28.9%（男28.3%、女30.8%）和2.8%（男2.4%、女3.8%）。未检出A、D、E、F、G、H基因型。结论:HBV感染者以B基因型为主。基因型B检出率显著高于其它基因型

其差异具有统计学意义（P<0.01）。HBV各基因型分布与性别无关（P>0.01）。

Abstract

Objective:To establish a set of suitable and reliable methods and to study the distribution of HBV genotypes.Methods:Type-specific primers were designed

and type-specific primers PCR was used to genotype the HBV strains.Results:A total of 218 out of the 238 strains were genotyped.Among them

49(68.3%) cases were genotype B(male 69.3%

female 65.3%)

63(28.9%) cases were genotype C(male 28.3%

female 30.8%)

6(2.8%) cases were mixtures of genotype B and C(male 2.4%

female 3.8%).No genotype A

G and H were found.Conclusion:Genotype B is the most dominant type for HBV strains and there is no relationship between the gender and HBV genotypes.

关键词

乙型肝炎病毒基因型型特异核苷酸聚合酶链反应

Keywords

hepatitis B virusgenotypeType-specific nucleotidespolymerase chain reaction(PCR)

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Related Institution

Department of Clinical Molecular Medicine of Children’s Hospital of Chongqing Medical University， National Clinical Research Center for Child Health and Disorders，Ministry of Education Key Laboratory of Child Development and Disorders，Chongqing Key Laboratory of Pediatrics

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Department of Infectious Diseases， Puren Hospital Affiliated to Wuhan University of Science and Technology

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