Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α

HUANG Xue-ping; et al

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Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α

更新时间：2024-01-17

- Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α
- Journal of Chongqing Medical University Issue 7, Pages: 814-816(2008)
- 作者机构：
  
  重庆医科大学基础医学院生物化学与分子生物学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q78
- Published：2008，
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[1]黄雪萍,王勇,罗耀玲,冉丹霞,马永平,宋方洲.产肠毒素大肠杆菌定居因子Ⅰ基因克隆与表达[J].重庆医科大学学报,2008(07):814-816.

HUANG Xue-ping, et al. Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α. [J]. Journal of Chongqing Medical University (7):814-816(2008)
[1]黄雪萍,王勇,罗耀玲,冉丹霞,马永平,宋方洲.产肠毒素大肠杆菌定居因子Ⅰ基因克隆与表达[J].重庆医科大学学报,2008(07):814-816. DOI：

HUANG Xue-ping, et al. Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5α. [J]. Journal of Chongqing Medical University (7):814-816(2008) DOI：

摘要

目的:在大肠杆菌DH5α中表达产肠毒素大肠杆菌（Enterotoxigenio escherichia coli

ETEC）定居因子CFA/Ⅰ

检测重组表达的ETEC CFA/Ⅰ。方法:以质粒pBV220为基础

构建pBV220-CFA/Ⅰ重组载体

表达产物用SDS-PAGE鉴定

免疫原性实验验证。结果:成功构建了pBV220-CFA/Ⅰ重组载体

表达产物的分子量与天然蛋白相同

动物免疫试验表明重组菌具有免疫原性。结论:CFA/Ⅰ基因的成功克隆为今后的ETEC CFA/Ⅰ候选疫苗研制奠定了基础。

Abstract

Objective:To clone and express colonization factor antigen Ⅰ(CFA/Ⅰ) of enterotoxigenic Escherichia coli (ETEC) in E.coli DH5α. Methods:CFA/Ⅰ gene was cloned by PCR from H10407 and recombined to a expression vector pBV220.This vector was designated pBV220- CFA/Ⅰand transformed into E.coli DH5α. The product was tested by SDS-PAGE and immunogenesis test. Results:The CFA/Ⅰ gene could be expressed in E.coli and contained strong immunogenesis by immune test. Conclusion:This study paved the way for using ETEC CFA/Ⅰ recombinant as a vaccine candidate in future.

关键词

产肠毒素大肠杆菌质粒pBV220定居因子抗原Ⅰ

Keywords

ETECpBV220CFA/Ⅰ

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