Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells

LUO Jun; et al

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Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells

更新时间：2024-01-17

- Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells
- Journal of Chongqing Medical University Issue 7, Pages: 817-820(2008)
- 作者机构：
  
  1. 四川省南充市中心医院消化内科
  2. 重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2008，
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[1]罗君,吴小翎.抗CXCR4单克隆抗体12G5对胃癌细胞粘附与侵袭的影响[J].重庆医科大学学报,2008(07):817-820.

LUO Jun, et al. Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells. [J]. Journal of Chongqing Medical University (7):817-820(2008)
[1]罗君,吴小翎.抗CXCR4单克隆抗体12G5对胃癌细胞粘附与侵袭的影响[J].重庆医科大学学报,2008(07):817-820. DOI：

LUO Jun, et al. Effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of gastric cancer cells. [J]. Journal of Chongqing Medical University (7):817-820(2008) DOI：

摘要

目的:探讨抗CXCR4单克隆抗体12G5对SGC7901细胞与基质的粘附性与侵袭性的影响。方法:应用MTT法、Transwell Chamber体外侵袭实验技术检测12G5（5μg/ml、10μg/ml、20μg/ml）对胃癌细胞株SGC7901粘附、侵袭和迁移能力的影响。结果:（1）10μg/ml的12G5即可使胃癌细胞粘附Matrigel的能力明显受抑

与对照组相比（P<0.01）。随着时间的延长及单抗浓度的加大

实验组的粘附能力低于对照组

差异有统计学意义（P<0.01）。（2）SGC7901细胞对照组和实验组侵袭迁移实验穿膜细胞数分别为:121.7±5.51

54.3±9.07;140.3±6.03

62.0±11.53。实验组的侵袭迁移能力明显低于对照组

差异有统计学意义（P<0.01）。结论:12G5能在一定程度上抑制SGC7901细胞的粘附性及侵袭性。

Abstract

Objective:To investigate the effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of SGC7901 cells.Methods:The effects of 12G5(5μg/ml

10μg/ml and 20μg/ml

respectively)on the adhesive、invasive and migrative abilities of SGC7901 cells were explored by MTT and Transwell chamber. Results:(1)12G5 showed a time/dose-dependent inhibition effect on SGC7901 cells adhering Matrigel

and the adhesion of gastric cancer cells was inhibited on the concentration of 10μg/ml of 12G5

and compared with untreated group

adhesion of SGC7901 cells was signficantly lower(P<0.01);(2)The invaded cell numbers in invasive and metastatic experiments of SGC7901 cell lines untreated or treated with 12G5 were 121.7±5.51

54.3±9.07;and 140.3±6.03

62.0±11.53 respectively

the invasive and metastatic abilities of treated group were lower than those of untreated group significantl(P<0.01). Conclusion:12G5 may inhibit adhesion and invasion of SGC7901 cells to certain extent.

关键词

SDF-1/CXCR4单克隆抗体SGC7901细胞细胞粘附细胞侵袭

Keywords

SDF-1/CXCR4monoclonal antibodySGC7901 cellscell adhesioncell invasion

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