Objectives:To evaluate the effect of hepatic VX2 tumor mode treated by radiofrequency ablation(RFA)

external high frequency thermotherapy(HFT) and transcatheter arterial chemoembolization(TACE).Methods:we replicated the model of hepatic tumor via transplantation and treated it with RFA

TACE and HFT.All the experimental rabbits was divided into four groups based on the different combination:group A:RFA+TACE+HFT

group B:RFA+HFT

group C:FA+TACE

Group D:TACE+HFT.Results:(1)After treatment

ALT and AST rised obviously on the first day in all groups

especially group A

which recovered the most slowly.In group B

however

ALT and AST was the lowest on the first day but decreased to normal most rapidly(P<0.05).(2)the gross tumor volume deflated significately after therapy in group A

group B and group C compared to that before the treatment(P<0.05)

while the gross tumor volume of group D increased obviously after treatment(P<0.05).(3)group A was able to kill more tumor cell and made tumor tissue localized better supported by pathological section exams.(4)proliferating cells nuclear antigen index(PI)was the lowest while apoptotic index(AI)and positive expression of HSP70 were the highest in group A.Conclusion:Group A can kill tumor cell and make tumor tissue localized more effectively

can activate the expression of Hsp70 better

which enhances the immunopotency of antitumors and leads to a better prognosis of tumor.But the combined therapeutic regimen also cause more fluctuation to liver function during the procedure.We should think considerately when applying the sequential therapy.