Expression and role of AQP9 in models of cultured steatosis hepatocytes

ZHAO Feng-ying; et al

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Expression and role of AQP9 in models of cultured steatosis hepatocytes

更新时间：2024-01-17

- Expression and role of AQP9 in models of cultured steatosis hepatocytes
- Journal of Chongqing Medical University Issue 8, Pages: 934-937(2008)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R575.5
- Published：2008，
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[1]赵丰莹,梅浙川.水通道蛋白9在培养肝细胞脂肪变性模型中的表达及意义[J].重庆医科大学学报,2008(08):934-937+1018.

ZHAO Feng-ying, et al. Expression and role of AQP9 in models of cultured steatosis hepatocytes. [J]. Journal of Chongqing Medical University (8):934-937(2008)
[1]赵丰莹,梅浙川.水通道蛋白9在培养肝细胞脂肪变性模型中的表达及意义[J].重庆医科大学学报,2008(08):934-937+1018. DOI：

ZHAO Feng-ying, et al. Expression and role of AQP9 in models of cultured steatosis hepatocytes. [J]. Journal of Chongqing Medical University (8):934-937(2008) DOI：

摘要

目的:探讨油酸诱导的脂肪变性肝细胞模型中水通道蛋白9（Aquaporin-9

AQP9）的表达及意义。方法:用油酸诱导正常肝细胞株L-02建立肝细胞脂肪变模型。采用油红O染色观察细胞内的脂滴

并检测细胞内甘油三脂

游离脂肪酸

甘油含量

RT–PCR法和Western blotting法分别检测AQP9 mRNA和蛋白的表达。结果:油红O染色显示24h开始出现脂肪变性

72h脂肪变性最重;模型组TG、FFA、甘油含量均在各时相点较对照组显著升高

差异有统计学意义（P<0.01）;模型组AQP9 mRNA的表达上调

于24h开始增高

72h表达最强

与对照组相比差异有统计学意义（P<0.05）;AQP9蛋白的表达24h开始增高

72h最强

与对照组相比差异有统计学意义（P<0.05）。结论:油酸诱导的脂肪变性肝细胞模型AQP9的表达增强

细胞内TG、FFA、甘油的合成增加

AQP9可能参与了肝细胞的脂代谢及脂肪变性的形成。

Abstract

Objective:To explore the expression and role of AQP9 in the models of cultured steatosis hepatocytes.Methods:Steatosis models of hepatocytes was established by adding oleic acid to the growing L-02 cell strain.The expression of AQP9 mRNA was measured with RT–PCR and the protein expression of AQP9 were measured by Western blotting.Lipid droplets in the hepatocytes were observed by oil red staining and the contents of triglyceride

FFA and glycerol in hepatocytes were measured with analyzed kit.Results:Through oil red staining.a few lipid droplets were observed at 24h and steatosis hepatocyte increased greatly at 72h.Triglyceride

FFA and glycerol contents in hepatocytes of model groups also increased

as compared with the control group(P<0.01).In model groups

mRNA expression of AQP9 increased at 24h

and elevated remarkably at 72h as compared with the normal control group(P<0.05)

and protein expression of AQP9 increased at 24h

and elevated remarkably at 72h compared with the control group(P<0.05).Conclusion:The overexpression of AQP9 in model groups could lead to disturbance of lipid metabolism and probably AQP9 participates the process of steatosis of hepatocytes.

关键词

油酸脂肪变肝细胞甘油三酯游离脂肪酸甘油AQP9

Keywords

Oleic acidSteatosis hepatocytetriglycerideFFAGlycerolAQP9

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