HAO Jian-wen, et al. The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry. [J]. Journal of Chongqing Medical University (8):947-950(2008)
HAO Jian-wen, et al. The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry. [J]. Journal of Chongqing Medical University (8):947-950(2008)DOI:
The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry
Objective:To study the expression of PEG-3 in cardiac muscle after electrical injury in order to find the time dependablity and the discrimination for the antemortem electrified and postmotorm electrified.Metheds:The animals were devided into two groups:the negative group and the experimental group.The experimental group were devided into three groups again:the electrical injury group
the electrouction group and the group of electrical shock after death.The electrical injury group is for 0h、3h、6h、12h、24h、3d、6d、12d
the electrouction group is for 0h、1h、3h、6h、12h
and the group of electrical shock after death is for 0h、0.5h、1h、3h
the control group is for 0h、0.5h、1h、3h、6h、12h.After all these we should get the cardiac tissue at the propotional time
and then dye it by the method of HE and immunohistochemistry.Then we can gain the conclusion through the method of semiquantitative analysis.Results:The expression of PEG-3 increased in heart injury early
it may be a useful molecular index to diagnose heart injury induced by electric force.In additional
the rule of PEG-3 expression was changed following time
which may help to estimate the injury interval in forensic EI field.We can also discriminate the the electrouction group and the postmotorm electrified group
because there are only extremely tenuous positive staining for the group of electrical shock after death.Conclusion:PEG-3 can diagnose the electrical injury and discriminate the electrical injury