Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP

YU Xiao-yan; et al

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Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP

更新时间：2024-01-17

- Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP
- Journal of Chongqing Medical University Issue 8, Pages: 951-953(2008)
- 作者机构：
  
  重庆医科大学附属第一医院耳鼻咽喉科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.6
- Published：2008，
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[1]余晓燕,陈鸿雁.TRAIL联合化疗药物诱导喉癌HEP-2细胞株凋亡的研究[J].重庆医科大学学报,2008(08):951-953+958.

YU Xiao-yan, et al. Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP. [J]. Journal of Chongqing Medical University (8):951-953(2008)
[1]余晓燕,陈鸿雁.TRAIL联合化疗药物诱导喉癌HEP-2细胞株凋亡的研究[J].重庆医科大学学报,2008(08):951-953+958. DOI：

YU Xiao-yan, et al. Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP. [J]. Journal of Chongqing Medical University (8):951-953(2008) DOI：

摘要

目的:探讨肿瘤坏死因子相关凋亡诱导配体（Tumor necrosis factor related apoptosis-inducingligand

TRAIL）与化疗药物氟尿嘧啶（5-FU）、顺铂（DDP）联合使用诱导喉癌HEP-2细胞株凋亡。方法:不同浓度的TRAIL、5-FU、DDP单独及TRAIL与5-FU、DDP联合处理HEP-2细胞

用四甲基偶氮唑蓝（MTT）法检测其细胞毒作用

荧光显微镜下观察并计算凋亡率

流式细胞仪（FCM）检测细胞凋亡率。结果:HEP-2细胞对低毒性的TRAIL不敏感

对化疗药物相对敏感

TRAIL与低毒性的化疗药物联合作用于细胞后可增强杀伤效应。结论:TRAIL与低毒性的5-Fu、DDP联用表现出高效的杀灭肿瘤细胞作用。

Abstract

Objective:To investigate whether TRAIL can synergize 5-Fu and DDP to kill HEP-2 cell line.Methods:MTT was used to detecet the cytotoxicity of HEP-2 cell line intervented in 5-FU

DDP

TRAIL

5-FU+TRAIL and DDP+TRAIL.Fluorescence microscope and FCM were used to detecet the apoptosis rate of cell.Results:HEP-2 cell line was insensitive in TRAIL

but the apoptosis rate obviously was upgraded after intervented in 5-FU+TRAIL and DDP+TRAIL.Conclusion:TRAIL in combination with subtoxic dose of 5-FU and DDP can effectively kill HEP-2 cell line.The combination of TRAIL

5-FU and DDP induced apoptosis of HEP-2 cell line.

关键词

TRAIL化疗HEP-2细胞株

Keywords

TRAILchemotherapyHEP-2 cell line

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Related Institution

Department of Urology，The First Affiliated Hospital of Chongqing Medical University

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Department of Infectious Disease,Yongchuan Hospital of Chongqing Medical University

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