Heredity effect on cord blood Th1/Th2 cells

ZHU Li-min; et al

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Heredity effect on cord blood Th1/Th2 cells

更新时间：2024-01-17

- Heredity effect on cord blood Th1/Th2 cells
- Journal of Chongqing Medical University Issue 7, Pages: 828-830(2008)
- 作者机构：
  
  重庆医科大学附属第一医院耳鼻咽喉科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R722.1
- Published：2008，
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[1]朱丽敏,钱迪,董小琴,洪苏玲.遗传因素对脐血Th1/Th2平衡的影响[J].重庆医科大学学报,2008(07):828-830.

ZHU Li-min, et al. Heredity effect on cord blood Th1/Th2 cells. [J]. Journal of Chongqing Medical University (7):828-830(2008)
[1]朱丽敏,钱迪,董小琴,洪苏玲.遗传因素对脐血Th1/Th2平衡的影响[J].重庆医科大学学报,2008(07):828-830. DOI：

ZHU Li-min, et al. Heredity effect on cord blood Th1/Th2 cells. [J]. Journal of Chongqing Medical University (7):828-830(2008) DOI：

摘要

目的:探讨遗传影响因素（父母有无过敏性疾病（Allergic diseases

AD）史）对新生儿脐血单个核细胞Th1/Th2平衡的影响。方法:取正常足月新生儿脐血为研究对象

其中20例父母有过敏性疾病史（AD组）和20例无过敏性疾病史（对照组）。收集脐血血浆并分离单个核细胞（CBMC）

体外经植物血凝素（PHA）刺激

MTT比色法检测细胞增殖活性

酶联免疫吸附试验（ELISA）检测培养48h后细胞培养上清液中IL-2、IL-4、IFN-r、GM-CSF的水平。结果:数据显示:AD组与对照组相比:（1）脐血血浆中IL-2、IL-4、GM-CSF有显著性差异（P﹤0.05）

IFN-r无显著性差异;（2）PHA干预培养48h后细胞培养上清夜中IL-2、IL-4、IFN-r、GM-CSF均有显著性差异（P<0.05）。结论:遗传因素与新生儿变应性疾病的发生有密切相关。

Abstract

Objective:To explore the effect of heredity on functional states of Thl/Th2 in cord blood mononuclear cells(CBMC)of neonates

whether their parents have anaphylactic disease. Methods:Cord blood was taken from 40 normal full term neonatals

who were divided into 20 ones with their parents having anaphylactic disease(AD group)

and 20 not(control group).Collect blood plasma and cord blood mononuclear cells(CBMC)of neonates were separated

Blood plasma was collected and after stimulation with PHA

cell proliferation was detected by MTT method. IL-2

IL-4

IFN-r

and GM-CSF in supernatant were assayed by ELASA after CBMCs were cultured in vitro for 48h. Results:In AD group

as compared with control group:(1)The expression of IL-2

IL-4 and GM-CSF in blood plasma were higher than those of control group(P<0.05)

but not IFN-r.(2)Cytokines’ expression of IL-2

IL-4

IFN-r

and GM-CSF were increased after PHA stimulation for 48 hours

with significant different from those in control group(P<0.05). Conclusion:Heredity effect may play an important role in generation and progression of newborn allergic diseases.

关键词

遗传脐血变应性疾病Th1/Th2

Keywords

heredityCord bloodAllergic diseasesThl/Th2

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