Comparative analysis of α-tubulin,β-tubulin and γ-tubulin of Immortalized cells and carcinoma cells of the Cervix

LV Yu-yu; et al

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Comparative analysis of α-tubulin,β-tubulin and γ-tubulin of Immortalized cells and carcinoma cells of the Cervix

更新时间：2024-01-17

- Comparative analysis of α-tubulin,β-tubulin and γ-tubulin of Immortalized cells and carcinoma cells of the Cervix
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1471-1474(2008)
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2008，
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[1]吕玉宇,赵涌,巫静娴,祝建芳,宋大萍.宫颈永生化细胞和癌细胞的α、β、γ–微管蛋白比较分析[J].重庆医科大学学报,2008,33(12):1471-1474.

LV Yu-yu, et al. Comparative analysis of α-tubulin,β-tubulin and γ-tubulin of Immortalized cells and carcinoma cells of the Cervix. [J]. Journal of Chongqing Medical University 33(12):1471-1474(2008)
[1]吕玉宇,赵涌,巫静娴,祝建芳,宋大萍.宫颈永生化细胞和癌细胞的α、β、γ–微管蛋白比较分析[J].重庆医科大学学报,2008,33(12):1471-1474. DOI：

LV Yu-yu, et al. Comparative analysis of α-tubulin,β-tubulin and γ-tubulin of Immortalized cells and carcinoma cells of the Cervix. [J]. Journal of Chongqing Medical University 33(12):1471-1474(2008) DOI：

摘要

目的:探讨中心体α、β、γ–微管蛋白在人鳞状上皮宫颈永生化细胞（H8细胞株）和癌细胞（Caski细胞株）的表达差异及其在宫颈癌发生发展过程中的作用。方法:采用免疫细胞化学SP法检测中心体α、β–微管蛋白在人宫颈永生化细胞和癌细胞中的表达差异

提取两株细胞的骨架蛋白并用Western blotting对α、β、γ–微管蛋白的表达水平进行半定量分析。结果:Caski细胞中α、β–微管蛋白表达水平高于H8细胞

差异均有统计学意义（P<0.05）。半定量分析α、β、γ–微管蛋白

Caski细胞中的表达量高于H8细胞。结论:中心体异常可能是宫颈癌细胞恶性表型的特征之一

以中心体微管蛋白为治疗靶点的研究有着可期待的前景。

Abstract

Objective:To investigate the expressions of α-tubulin

β-tubulin and γ-tubulin of centrosome in immortalized hu-man squamous epithelial cel(lH8)and cervical cancer cel(lCaski)and the role of them in occurrence and development of cer-vical cancer.Methods:Immunocytochemistry was used to analyze the protein expressions of α-tubulin and β-tubulin in im-mortalized human cervical cells and carcinoma cells.The skeleton protein was extracted by ProteoExtract Subcellular Proteome Extraction Kit

and then Western blotting was used to determine the protein expressions of α-tubulin

β-tubulin and γ-tubulin semi-quantitatively.Results:The protein expressions of α–tubulin

β-tubulin and γ-tubulin in Caski were higher than those in H8(P<0.05).Conclusion:Centrosome abnormality may be one of the malignant phenotypes of cervical cancer cells.The micro-tubulin protein as a target in tumor treatment can have a good perspective.

关键词

宫颈癌永生化微管蛋白中心体

Keywords

cervical carcinomaimmortalizationMicrotubulinCentrosome

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