Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell

SONG Da-ping; et al

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Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell

更新时间：2024-01-17

- Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell
- Journal of Chongqing Medical University Issue 2, Pages: 166-168(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学基础医学院病理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2008，
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[1]宋大萍,赵涌,巫静娴,贾世军.宫颈永生化细胞和癌细胞的胞质蛋白双向电泳图谱的建立及初步分析[J].重庆医科大学学报,2008(02):166-168+176.

SONG Da-ping, et al. Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell. [J]. Journal of Chongqing Medical University (2):166-168(2008)
[1]宋大萍,赵涌,巫静娴,贾世军.宫颈永生化细胞和癌细胞的胞质蛋白双向电泳图谱的建立及初步分析[J].重庆医科大学学报,2008(02):166-168+176. DOI：

SONG Da-ping, et al. Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell. [J]. Journal of Chongqing Medical University (2):166-168(2008) DOI：

摘要

目的:建立HPV-16阳性的宫颈上皮永生化细胞（H8细胞株）和宫颈癌细胞（CaSKi细胞株）胞质蛋白双向电泳图谱

并初步分析找出特征性的差异蛋白点。方法:分别培养H8和CaSki细胞

用亚细胞结构蛋白质提取试剂盒提取胞质蛋白进行双向电泳

用ImageMaster 2D V2002.1图像分析软件分析电泳图谱

筛选差异蛋白点。结果:获得了分辨率和重复性均较好的宫颈永生化细胞和癌细胞胞质蛋白2-DE图谱

并分析找出了显著的差异蛋白点8个

其中3种蛋白质在宫颈癌细胞株表达上调

1种蛋白质表达明显下调

2种蛋白质在宫颈癌细胞株表达缺失

2种蛋白质在永生化细胞株表达缺失。结论:应用亚细胞蛋白双向凝胶电泳技术对HPV-16阳性的宫颈癌前和癌变的胞质蛋白分析

能有效找出蛋白质表达差异点

为进一步确定宫颈癌前病变和癌细胞的关键性差异蛋白质的结构和功能奠定了基础。

Abstract

Objective:To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from immortalized human endocervical cell(H8) and cervical cancer cell(CaSKi)

and to identify the differential expressions of cytoplasmic proteins.Methods:H8 cells and CaSKi cells were incubated

the cytoplasmic proteins of H8 cells and CaSKi cells were extracted and seperated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The differential expression proteins were analyzed by using ImageMaster 2D analysis software.Results:We obtained well-resolved

reproducible 2-DE patterns

and 8 notable differential protein spots were defined in 2-DE gels.Conclusion:Cytoplasmic proteins of cervical precancerous lesion and cervical cancer are separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The expression maps of proteomics in cytoplasmic proteins of H8 and CaSKi are established.There are significant differences in proteome between cytoplasmic proteins of H8 cells and CaSKi cells.These data may be valuable for differential proteomics research of cervical precancerous lesion and cervical cancer.

关键词

宫颈永生化细胞癌细胞蛋白质组2-DE

Keywords

cervixHuman immortalized endocervical cellCancer cellProteomicsTwo-dimensional gel electrophoresis(2-DE)

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