The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2

GONG Yu; et al

您当前的位置：

首页 >

文章列表页 >

The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2

更新时间：2024-01-17

- The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1479-1484(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学附属第一医院神经病学实验室
  3. 重庆市第九人民医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.65
- Published：2008，
扫描看全文
[1]龚宇,胡国华,汤为学,董蒲江,黄春蓓.NRP-1对喉癌Hep-2细胞增殖侵袭及体外癌细胞促血管形成的影响[J].重庆医科大学学报,2008,33(12):1479-1484.

GONG Yu, et al. The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2. [J]. Journal of Chongqing Medical University 33(12):1479-1484(2008)
[1]龚宇,胡国华,汤为学,董蒲江,黄春蓓.NRP-1对喉癌Hep-2细胞增殖侵袭及体外癌细胞促血管形成的影响[J].重庆医科大学学报,2008,33(12):1479-1484. DOI：

GONG Yu, et al. The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2. [J]. Journal of Chongqing Medical University 33(12):1479-1484(2008) DOI：

摘要

目的:探讨NRP-1对喉癌Hep-2细胞株的增殖迁移、侵袭及体外血管形成的功能的影响

为研究NRP-1在喉癌发生发展过程中的作用提供依据。方法:用MTT法、细胞运动实验、细胞侵袭实验及体外血管生成实验分别检测NRP-1抗体组及对照组Hep-2细胞株增殖、粘附迁移、侵袭能力及体外促血管形成的能力并进行比较。结果:NRP-1抗体干预组Hep-2细胞增殖抑制率随NRP-1抗体浓度的增加而升高（r=0.624

P<0.01）。NRP-1抗体处理48h后细胞迁移能力减弱、细胞粘附能力明显减弱、侵袭力明显下降

均与对照组差异有显著性（P<0.01）

在体外基质胶上诱导血管细胞形成的管腔也明显减少（P<0.01）。结论:NRP-1抗体有效抑制了喉癌Hep-2细胞株的增殖、粘附、迁移、侵袭能力及癌细胞促体外血管形成的能力

证实NRP-1不仅在肿瘤血管生成中起到重要作用

其对癌细胞自身增殖及迁移也有显著影响

提示NRP-1在喉癌发生发展过程中起到重要作用

拮抗NRP-1可能成为肿瘤治疗新途径。

Abstract

objective:To identify the function of NRP-1 in progression and angiogenesis of laryngocarcinoma cell line Hep-2 in vitro.methods:MTT

the cell migrate

attack and vascular formation experiments in vitro was used to evaluate the effect of NRP-1 antigen on the migrate

attack and angiogenesis capability of laryngocarcinoma cell line Hep-2.Results:The proliferation inhibition ratio of Hep-2 cells was risen up as the NRP-1 antigen’s concentration was increaced(r=0.624

P<0.01).The number of cells migrated in the group with NRP-1 antigen was fewer than that in the control group(P<0.01).After Hep-2 cells were treated with NRP-1 antigen for 48 hours

compared with the control group

the adhesion

invasion ability was significantly weakened(P<0.01)and induction of vessel lumina formation on matrigel was much less in vitro(P<0.01).Conclustion:The NRP-1 antigen suppressed the Hep-2 cell line’s proliferation

adhesion

migration

invasion

and angiogenesis in vitro

confirmed that NRP-1 not only played an impotant role in vascular endothelial cell angiogenesis

but also had remarkable influence on Hep-2 cells.It suggested that NRP-1 may plays an impotant role in the laryngocarcinoma’s tumorigenesis and developing process

inhibit the expression of NRP-1 may be a new stratage in the treatment of laryngocarcinoma

关键词

喉肿瘤神经纤毛蛋白-1肿瘤形成血管生成

Keywords

Laryngeal carcinomaneuropilin-1TumorigenesisAngiogenesis.

references

Views

134

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective effect of neuropilin-1 overexpression on isoproterenol-induced heart failure in rats

Related Author

Jiang Fangping

Related Institution

Department of Cardiovascular Medicine, The 363th Hospital

⁰