Study on the postmortem stability of heart fatty-acid-binding protein for the diagnosis of early myocardial infraction

GUO Miao; et al

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Study on the postmortem stability of heart fatty-acid-binding protein for the diagnosis of early myocardial infraction

更新时间：2024-01-17

- Study on the postmortem stability of heart fatty-acid-binding protein for the diagnosis of early myocardial infraction
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1488-1491(2008)
- 作者机构：
  
  重庆医科大学基础医学院法医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R542.22
- Published：2008，
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[1]郭淼,万立华.心肌脂肪酸结合蛋白在诊断早期心肌梗死的死后稳定性研究[J].重庆医科大学学报,2008,33(12):1488-1491.

GUO Miao, et al. Study on the postmortem stability of heart fatty-acid-binding protein for the diagnosis of early myocardial infraction. [J]. Journal of Chongqing Medical University 33(12):1488-1491(2008)
[1]郭淼,万立华.心肌脂肪酸结合蛋白在诊断早期心肌梗死的死后稳定性研究[J].重庆医科大学学报,2008,33(12):1488-1491. DOI：

GUO Miao, et al. Study on the postmortem stability of heart fatty-acid-binding protein for the diagnosis of early myocardial infraction. [J]. Journal of Chongqing Medical University 33(12):1488-1491(2008) DOI：

摘要

目的:探讨心肌脂肪酸结合蛋白（Heart fatty-acid-binding protein

H-FABP）诊断早期心肌梗死的死后诊断的稳定性。方法:建立兔急性心肌缺血模型

应用免疫组化和图像分析技术

检测死后不同时间缺血心肌细胞内H-FABP脱失面积变化规律。结果:正常心肌组织4℃放置1～2d H-FABP染色均匀未见明显脱失

放置3d以上

可见H-FABP脱失

且随放置时间的延长

脱失面积逐步增大。缺血心肌组织随放置时间的延长

脱失面积逐步增大

图像分析结果显示4d以内缺血心肌组织胞浆内H-FABP的脱失面积与正常对照组比较差异有统计学意义（P<0.01）

放置超过5d以上

其脱失面积与正常对照组比较差异无统计学意义。结论:H-FABP在心肌梗死死后诊断的稳定性一般

受自溶的影响较小可用于死后4℃放置的4d内尸体的检测

在法医学实践中具有一定的实用价值。

Abstract

Objective:To investigate the stability of heart fatty-acid-binding protein(H-FABP)for the postmortem diagnosis of early myocardial infraction.Methods:Rabbit model of myocardial ischemia was induced by ligating the anterior branch of the left coronary artery.The areas of H-FABP depletion in the ischemic myocardial cells were studied by immunohistochemistry

image analysis technique and statistical system.Results:The specimens of normal myocardium kept at 4℃ for 1 to 2 days showed homogenous brown reactions for H-FABP.The depletion of H-FABP became evident when these specimens were kept at 4℃ for 3 days after death

and the depletion area increased with the lapse of postmortem interval.The depletion area of H-FABP in ischemic myocardial tissue also increased with the lapse of postmortem interval.The results of image analysis indicated that the depletion area of H-FABP in ischemic myocardial tissue within 4 d were significantly different from that in the normal control group(P<0.01)

while within 5 d the depletion area of H-FABP in the two groups was not significantly differen(tP>0.05).Conclusion:The H-FABP shows medium postmortem stability

and small influenced by autolysis

which is suitable for detection in corpses at 4℃ 4 days after death.H-FABP as a marker for postmortem diagnosis of AMI has some practical value in forensic medicine.

关键词

早期心肌梗死心肌脂肪酸结合蛋白稳定性

Keywords

Early myocardial infractionHeart fatty-acid-binding proteinstability

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