Preliminary study on the effect of synthetic antimicrobial peptides on Escherichia coli biofilm

XU Yan-an; et al

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Preliminary study on the effect of synthetic antimicrobial peptides on Escherichia coli biofilm

更新时间：2024-01-17

- Preliminary study on the effect of synthetic antimicrobial peptides on Escherichia coli biofilm
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1492-1494(2008)
- 作者机构：
  
  重庆医科大学附属第一医院骨科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R378.21
- Published：2008，
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[1]徐炎安,张健,周爱国.人工合成抗菌肽对大肠杆菌生物膜作用的初步研究[J].重庆医科大学学报,2008,33(12):1492-1494.

XU Yan-an, et al. Preliminary study on the effect of synthetic antimicrobial peptides on Escherichia coli biofilm. [J]. Journal of Chongqing Medical University 33(12):1492-1494(2008)
[1]徐炎安,张健,周爱国.人工合成抗菌肽对大肠杆菌生物膜作用的初步研究[J].重庆医科大学学报,2008,33(12):1492-1494. DOI：

XU Yan-an, et al. Preliminary study on the effect of synthetic antimicrobial peptides on Escherichia coli biofilm. [J]. Journal of Chongqing Medical University 33(12):1492-1494(2008) DOI：

摘要

目的:研究人工合成抗菌肽对大肠杆菌生物膜的影响。方法:采用试管法建立大肠杆菌生物膜

将大肠杆菌生物膜随机分成4个组A组:样本中加入4×MIC抗菌肽;B组样本中加入1×MIC庆大霉素;C组样本中联合加入4×MIC抗菌肽和1×MIC庆大霉素;D组样本中加入PBS液作为空白对照。处理6h后对样本进行活菌计数

并利用扫描电镜观察比较处理组与对照组间差异。结果:6h后活菌计数

C组与其他三组相比活菌数明显减少（P≤0.05）;而A组和B组与D组间比较活菌数减少（P≤0.05）。扫描电镜观察可见A组与C组中生物膜内大肠杆菌的细胞膜完整性受到破坏。结论抗菌肽与庆大霉素联合运用时的杀菌效果显著增强。

Abstract

Objective:To study the effect of synthetic antimicrobial peptides on Escherichia coli biofilm.Methods:The test tube method was used to establish the model of Escherichia coli biofilm.The samples were randomly divided into four groups:group A

by adding 4×MIC antimicrobial peptides to the samples

group B

by adding 1×MIC gentamicin to the samples

group C

by adding 4×MIC antimicrobial peptides and 1×MIC gentamicin to the samples

and group D

by adding phosphate buffered saline to the samples as a control.After 6 h the viable bacteria in the biofilm were counted following the method of colony count.The different changes in the biofilm were compared between the treated groups and the control group with scanning electron microscope.Results:The viable bacteria in group C were significantly decreased compared with the other three groups(P≤0.05).While compared with group D

the viable bacteria were decreased in group A and group B(P≤0.05).From the graph of scanning electron microscope the integrity of the Escherichia coli cell membrane was disrupted in group A and C.Conclusion:The results suggest that the killing effect of combination of antimicrobial peptides with gentamicin is significantly increased.

关键词

抗菌肽庆大霉素生物膜大肠杆菌

Keywords

Antimicrobial peptidesgentamicinbiofilmescherichia coli

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