Effects of CO<sub>2</sub> pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi

LI Gang; et al

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Effects of CO₂ pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi

更新时间：2024-01-17

- Effects of CO₂ pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi
- Journal of Chongqing Medical University Issue 7, Pages: 788-791(2008)
- 作者机构：
  
  重庆医科大学附属第一医院妇产科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2008，
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[1]李刚,姚珍薇,耿力.CO_2气腹对人宫颈癌CasKi细胞p-ERK和p-Akt表达的影响[J].重庆医科大学学报,2008(07):788-791.

LI Gang, et al. Effects of CO₂ pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi. [J]. Journal of Chongqing Medical University (7):788-791(2008)
[1]李刚,姚珍薇,耿力.CO_2气腹对人宫颈癌CasKi细胞p-ERK和p-Akt表达的影响[J].重庆医科大学学报,2008(07):788-791. DOI：

LI Gang, et al. Effects of CO₂ pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi. [J]. Journal of Chongqing Medical University (7):788-791(2008) DOI：

摘要

目的:探讨CO2气腹对人宫颈癌CasKi细胞信号转导通路的影响。方法:建立腹腔镜CO2气腹体外模型

以不同压力（12mmHg、13mmHg及14mmHg）的CO2气腹对人宫颈癌CasKi细胞处理2h

设未处理组为对照组。采用四唑盐比色试验（MTT）、免疫细胞化学法、Western blotting检测CasKi细胞体外生长情况、相关信号转导通路核心蛋白p-ERK和p-Akt的表达水平。结果:CO2处理组中CasKi细胞增殖加快

相关信号转导通路核心蛋白p-ERK和p-Akt的表达显著增加（P<0.01）。各个CO2处理组间

CasKi细胞增殖及p-ERK与p-Akt的表达无显著差异（P>0.05）。结论:在临床常用腹腔镜气腹压力和作用时间范围内

CO2气腹激活相关信号转导通路

这可能是CO2气腹促肿瘤细胞生长机制之一。

Abstract

Objective:To study the effects of CO

pneumoperitoneum on the signal transduction pathways of human cervical carcinoma cell line CasKi. Methods:The imitated CO

pneumoperitoneum model was established in vitro. After CasKi cells were exposed to CO

pneumoperitoneum at 12

13 or 14mmHg for 2h

the cell growth and expressions of phosphor-ERK and phosphor-Akt were examined by MTT

immunocytochemistry and Western blotting respectively. The untreated CasKi cells served as control. Results:After treatment of CO

pneumoperitoneum

the proliferation of human cervical carcinoma cell line CasKi significantly increased

and the expressions of phosphor-ERK and phosphor-Akt also significantly increased（P

0.01）. Among the CO

treated groups

the proliferation of human cervical carcinoma cell line CasKi and the expressions of phosphor-ERK and phosphor-Akt were not observed significant difference（P

0.05）. Conclusion:Under the pressure and during the exposure time which were often used in operational laparoscopy

pneumoperitoneum could activate correlated signal transduction pathways

which may be one reason for the promoting effect of CO

pneumoperitoneum on malignant cells growth.

关键词

二氧化碳气腹宫颈肿瘤信号转导

Keywords

Carbon dioxidepneumoperitoneumCervical neoplasmSignal transduction

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Related Institution

重庆医科大学附属第一医院妇产科

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⁰

Effects of CO2 pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi

DOI：

摘要

Abstract

关键词

Keywords

references

Effects of CO₂ pneumoperitoneum on expressions ofp-ERK and p-Akt in human cervical carcinoma cell line CasKi