Comparison of distribution of single nucleotide polymorphism in normal mandible and protruding mandible

WANG Yu-rong; et al

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Comparison of distribution of single nucleotide polymorphism in normal mandible and protruding mandible

更新时间：2024-01-17

- Comparison of distribution of single nucleotide polymorphism in normal mandible and protruding mandible
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1522-1524(2008)
- 作者机构：
  
  重庆医科大学附属口腔医院口腔正畸科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R783.5
- Published：2008，
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[1]王豫蓉,余兴华,戴红卫,张定铭,陈允嘉,李文颖.生长激素受体基因单核苷酸多态性在下颌前突与下颌正常人群中的分布比较[J].重庆医科大学学报,2008,33(12):1522-1524.

WANG Yu-rong, et al. Comparison of distribution of single nucleotide polymorphism in normal mandible and protruding mandible. [J]. Journal of Chongqing Medical University 33(12):1522-1524(2008)
[1]王豫蓉,余兴华,戴红卫,张定铭,陈允嘉,李文颖.生长激素受体基因单核苷酸多态性在下颌前突与下颌正常人群中的分布比较[J].重庆医科大学学报,2008,33(12):1522-1524. DOI：

WANG Yu-rong, et al. Comparison of distribution of single nucleotide polymorphism in normal mandible and protruding mandible. [J]. Journal of Chongqing Medical University 33(12):1522-1524(2008) DOI：

摘要

目的:研究生长激素受体基因（Growth hormone receptor

GHR）的单核苷酸多态性（Single nucleotide polymorphism

SNP）在前突下颌（实验组）与正常下颌（对照组）中分布有无差异。方法:依照纳入标准从2005～2006年重庆医科大学附属口腔医院就诊的患者中筛选出187例受试者（男86例

女101例）

抽取静脉血液5ml

提取基因组DNA

PCR扩增目的基因、测序

将实验测得序列与美国国家生物科技资讯中心（National Center for Biotechnology Information

NCBI）中公布的基因序列对比

最后采用SPSS10.0统计软件对实验组与对照组中基因型与等位基因频率进行χ2检验统计分析。结果:在实验组与对照组中都只发现1673这1个SNP位点

在该位点上存在A、C2种碱基及AC、CC、AA3种基因型

其基因型与基因频率分布在实验组与对照组中均存在差异（P<0.01）。结论:实验结果提示GHR的SNP在下颌正常与下颌前突人群中的分布可能存在差异

但需要更多的标本量进一步证实。

Abstract

Objective:To study the differences of growth hormone recepto(rGHR)gene between normal mandible(control group)and protruding mandible(study group)in molecular level by comparing the gene’s nucleotide sequences.Methods:In our study

we selected 100 individuals with protruding mandibles and 87 individuals with normal mandibles by the study criterions

diagnosed in Affiliated Stomatological Hospital of Chongqing Medical University during 2005～2006.5 ml venous blood was withdraw and the genomic DNA was isolated from the whole blood cells with the use of a DNA Blood Kit.PCR amplification was performed by a standard protocol to increase and sequence the aim gene in normal and protruding individuals.And then we compared the acquired gene sequences with the published ones in NCBI(national center for biotechnology information)database

finally found the positions of single nucleotide polymorphism(SNP)in exon 10 of GHR gene and calculated the allele frequency and genotype frequency in study and control groups by SPSS10.0.Results:One SNP position(including study and control groups)was found in our study and his nucleotide code was 1673.In this SNP position

there were two alleles(AC)and three genotypes(AC

AA and CC).The allele frequency and distributions of genotype had a significant difference by χ2 calculation in 0.01 level.Conclusion:Differences in sequences of growth hormone receptor gene exist in individuals with protruding mandibles and individuals with normal mandibles.

关键词

生长激素受体单核苷酸多态性正常下颌前突下颌

Keywords

growth hormoneReceptorsingle nucleotide polymorphismNormal mandibleProtruding mandible

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