Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts

LUO Hong-zhi

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Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts

更新时间：2024-01-17

- Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1505-1508(2008)
- 作者机构：
  
  湖南省怀化市第一人民医院外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R6221
- Published：2008，
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[1]罗洪智.外源性一氧化氮对体外培养瘢痕成纤维细胞的抑制作用[J].重庆医科大学学报,2008,33(12):1505-1508.

LUO Hong-zhi. Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts. [J]. Journal of Chongqing Medical University 33(12):1505-1508(2008)
[1]罗洪智.外源性一氧化氮对体外培养瘢痕成纤维细胞的抑制作用[J].重庆医科大学学报,2008,33(12):1505-1508. DOI：

LUO Hong-zhi. Exogenous nitric oxide inhibits proliferation of in vitro cultured human keloid fibroblasts. [J]. Journal of Chongqing Medical University 33(12):1505-1508(2008) DOI：

摘要

目的:探讨外源性一氧化氮对体外培养瘢痕成纤维细胞增殖的抑制作用。方法:取我院整形外科9例病理性瘢痕组织标本

组织块贴壁法培养瘢痕成纤维细胞。传代细胞分5组

对照A组不加一氧化氮供体硝普钠

实验组B～D组加入浓度分别为100、200、300μmol/L的硝普钠

实验组E组加入浓度为200μmol/L的硝普钠及和10μmol/L的鸟苷酸环化酶抑制剂美蓝。24h后用Griess还原法检测细胞上清液中一氧化氮的释放量

并进行MTT试验。结果:病理性瘢痕组织标本原代培养成纤维细胞全部成活

形态正常。实验24h后细胞上清液中一氧化氮释放量随硝普钠浓度的增高而增加。MTT试验显示经硝普钠作用后瘢痕成纤维细胞的活细胞数较对照组明显减少（P<0.05）

实验组B～D之间

随着硝普钠浓度的增高

细胞活力也有降低趋势。并且

加入美蓝的实验组E较实验组C活细胞数增高（P<0.05）。免疫细胞化学检测显示:SNP作用后Ⅰ型胶原表达降低

而美蓝作用后Ⅰ型胶原表达增加。Ⅲ型胶原检测结果也有相同的变化。结论:外源性一氧化氮对培养状态下瘢痕成纤维细胞有抑制作用

这其中可能有NO-GC-cGMP通路的参与。

Abstract

Objective:To explore the inhibitive effects of exogenous nitric oxide on the proliferation of in vitro cultured human keloid fibroblasts.Methods:Fibroblasts was isolated from fresh pathological scar tissue and cultured.Fibroblasts were distributed in five groups.Fibroblasts in control group A was cultured without SNP;in experiment group B～D

SNP was used in the concentration of 100、200、300 μmol/L each;in experiment group E

Fibroblasts was cultured with 200 μmol/L SNP and 10 μmol/L methylene blue.After 24h

Griess and MTT were used to detect the concentration of NO and cell vigor.Results:The release of NO increased along with the enhancement of the concentration of SNP.The amount of living cells in experiment groups decreased compared with the controls as shown by MTT tes(tP<0.05)

but in experiment group E

the amount of living cells was increased than group C(P<0.05).Immunocytochemical detection shows:SNP treatment decreased the expression of collagen Ⅰ

and expression of collagen Ⅰ increased after treated by Methylene blue.Conclusion:Exogenous nitric oxide inhibits the proliferation of of in vitro cultured human keloid fibroblasts

and these may be result in NO-GC-cGMP path way.

关键词

病理性瘢痕成纤维细胞一氧化氮细胞增殖细胞培养

Keywords

pathological scarfibroblastnitric oxidecell proliferationIn vitro

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Related Institution

Key Laboratory of Molecular Biology of Infectious Diseases Designated by the Chinese Ministry of Education， Chongqing Medical University

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Department of Anesthesiology,The First Affiliated Hospital of Chongqing Medical University

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