Expressions of HMGB1mRNA and NF-κB in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin

YANG Ting-fang; et al

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Expressions of HMGB1mRNA and NF-κB in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin

更新时间：2024-01-17

- Expressions of HMGB1mRNA and NF-κB in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin
- Journal of Chongqing Medical University Vol. 34, Issue 1, Pages: 24-28(2009)
- 作者机构：
  
  重庆医科大学附属儿童医院ICU
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285;R574
- Published：2009，
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[1]杨廷芳,王兴勇,胡语航,徐静,熊燚,周海银,何蓉,许峰.NF-κB和HMGB1在大鼠肠缺血再灌注肺损伤中的表达及白藜芦醇苷的保护作用[J].重庆医科大学学报,2009,34(01):24-28.

YANG Ting-fang, et al. Expressions of HMGB1mRNA and NF-κB in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin. [J]. Journal of Chongqing Medical University 34(1):24-28(2009)
[1]杨廷芳,王兴勇,胡语航,徐静,熊燚,周海银,何蓉,许峰.NF-κB和HMGB1在大鼠肠缺血再灌注肺损伤中的表达及白藜芦醇苷的保护作用[J].重庆医科大学学报,2009,34(01):24-28. DOI：

YANG Ting-fang, et al. Expressions of HMGB1mRNA and NF-κB in lung injury induced by intestinal ischemia-reperfusion in rats and protective effects of polydatin. [J]. Journal of Chongqing Medical University 34(1):24-28(2009) DOI：

摘要

目的:探讨肠缺血再灌注（Intestinal ischemia-reperfusion

IIR）肺损伤核转录因子-κB（Nuclearfactor-kappaB

NF-κB）的活性和高迁移率族蛋白1（High mobility group box1

HMGB1）基因表达及白藜芦醇苷（Polydatin

PD）的保护作用。方法:54只Sprague-Dawley（SD）大鼠随机分成对照组、肠缺血45min继以再灌注组（再灌注0.5h、2h、6h）及肠缺血45min继以再灌注同时给予PD治疗组（再灌注0.5h、2h、6h）。采用比色法检测肺组织髓过氧化物酶（myeloperoxidase

MPO）水平、免疫组化法检测细胞间黏附分子-1（Intercellular adhesion molecule-1

ICAM-1）和NF-κB的表达、RT-PCR检测肺组织高迁移率族蛋白1（HMGB1）mRNA。结果:IIR后肺组织明显水肿、出血和炎性细胞侵润。与对照组相比

肺组织W/D、MPO水平明显升高

ICAM-1及NF-κB的蛋白表达

HMGB1mRNA（2h、6h）的表达增强（P<0.05）;白藜芦醇苷干预后肺损伤程度减轻、各项指标明显改善（P<0.05）。结论:PD可以减轻肠缺血再灌注肺损伤

作用机制可能与其抑制NF-κB活化

降低肺组织HMGB1mRNA的表达上调密切相关。

Abstract

Objective:To evaluate the activity of nuclear factor kappaB(NF-κB)and the gene expression of high mobility group box 1(HMGB1)in lung injury induced by intestinal ischemia-reperfusion(IIR)

and to investigate the protective effect of polydatin(PD)in this lung injury.Methods:Fifty-four male Sprague-Dawley rats weighing 200~250 g were randomly divided into control group

IIR group(45 min intestinal ischemia with 0.5 h or 2 h or 6 h reperfusion)and PD group [IIR with PD(10 mg/kg)treatment].The level of pulmonary myeloperoxidase(MPO)was detected by colorimetric method

the expressions of NF-κB and ICAM-1 by immuneochemistry and that of pulmonary HMGB1mRNA by RT-PCR respectively.Results:Lung injury induced by IIR was characterized by pathological damage of edema

hemorrhage and inflammatory cell infiltration.Compared with control group

pulmonary W/D and MPO increased significantly(P<0.05).Positive expressions of NF-κB p65 and ICAM-1 protein

and HMGB1mRNA(2 h、6 h)also increased significantly(P<0.05).After administration of PD

the levels of pulmonary indices were improved significantly(P<0.05)

pulmonary damage alleviated compared with IIR group(P<0.05)

and the expresion of NF-κB became weak.Conclusion:Injection of polydatin before reperfusion could alleviated lung injury induced by intestinal ischemia-reperfusion in rats in vivo

which is possibly through inhibiting pulmonary NF-κB activation and attenuating the expression of HMGB1mRNA during lung injury in rats.

关键词

肠缺血再灌注肺损伤核转录因子-κB高迁移率族蛋白1白藜芦醇苷

Keywords

Intestinal ischemia-reperfusion(IIR)lung injuryNuclear factor-kappaB(NF-κB)High mobility group box1(HMGB1)Polydatin(PD)

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