Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes

SONG Jin; et al

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Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes

更新时间：2024-01-17

- Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes
- Journal of Chongqing Medical University Issue 11, Pages: 1334-1337(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院神经内科
  2. 浙江省衢州市衢化医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.3
- Published：2008，
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[1]宋进,张杰敏,陈阳美.HSP70被抗体封闭后对星形胶质细胞损伤后BDNF表达的影响[J].重庆医科大学学报,2008(11):1334-1337.

SONG Jin, et al. Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes. [J]. Journal of Chongqing Medical University (11):1334-1337(2008)
[1]宋进,张杰敏,陈阳美.HSP70被抗体封闭后对星形胶质细胞损伤后BDNF表达的影响[J].重庆医科大学学报,2008(11):1334-1337. DOI：

SONG Jin, et al. Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes. [J]. Journal of Chongqing Medical University (11):1334-1337(2008) DOI：

摘要

目的:探讨用抗体封闭热休克蛋白70（Heat shock protein70

HSP70）后对星形胶质细胞损伤后表达脑源性神经营养因子（Brain-derived neurotropic factor

BDNF）的影响。方法:原代培养并纯化星形胶质细胞

采用划痕损伤法构建细胞损伤模型

通过培养液内加入HSP70抗体来进行干预。在不同时间点对对照组和损伤干预组进行BDNF免疫细胞化学染色

观察细胞增生情况及BDNF的表达强度

并用RT-PCR检测BDNFmRNA的表达水平。结果:与对照组相比

干预组BDNF的表达明显减弱（P<0.05

P<0.01）

GFAPmRNA的表达也明显降低（P<0.05

P<0.01）。结论:星形胶质细胞损伤后

HSP70可能具有促进星形胶质细胞表达BDNF的作用。

Abstract

Objective:To study its effect on the expression of BDNF from injured astrocytes after HSP70 were blocked by HSP70-antibody. Methods:We performed scratch injuries in the cultured rat astrocytes

and added HSP70-antibody into the nu- trient medium at once after injury(intervention group).Then

at 30min

24h

7d after injury

the expression of brain-derived neurotropic facto(rBDNF)was studied by immunocytochemical and reverse transcriptase-polymerase chain reaction(RT-PCR).The immunocytochemical pictures were analyzed by image analysis system to abtain the average optical density of BDNF. Results: The expression of BDNF increased gradually with injury time.Compared with the control group

the average optical density of BDNF in the intervention group were significantly reduced(P<0.05

P<0.01).The expresstion of BDNFmRNA was down-regu- lated in the intervention group(P<0.05

P<0.01). Conclusion:HSP70 may play a role in the production of BDNF from injured astrocytes.

关键词

星形胶质细胞划痕损伤热休克蛋白70抗体脑源性神经营养因子

Keywords

astrocyteScratch injuriesHeat shock protein70(HSP70)antibodyBrain-derived neurotropic facto(rBDNF)

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